Immobilized biological additive and its preparation method and uses
A technology of biological additives and additives, applied in the fields of application, tobacco, and tobacco treatment, can solve the problems of occupying large warehouses, overstocked funds, and the quality of smoking is not as good as that of natural alcoholized tobacco leaves, so as to reduce diffusion, reduce organic macromolecular substances, and be good The effect of developing application prospects
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Embodiment 1
[0023] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, ethyl stearate 0.5%, potassium tartrate 0.05 %, sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate added to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.
Embodiment 2
[0025] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, behenyl alcohol stearate 0.5%, tartaric acid Potassium 0.05%, sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate added to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.
Embodiment 3
[0027] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, triglyceride 0.5%, potassium tartrate 0.05%, Sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate add up to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.
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