Polysaccharose MF4 of mussel with enhancing immunity and anti-tumour activity

Abstract

Glycan ingredient MF4 has increased immunological competence and anti-tumour functions, which is separated from sea-animal thick shell mussel. Its relative molecular weight is 113,000. It was analyzed and identified the physical properties and chemical structure through multiple spectra and methods, of which spectra are such as UV, IR, GC / MS,1HNMR, of which chemical methods are such as hydrolysis, acetylization, periodate oxidation, Smith degradation, methylation, sulfuric acid- carbazole reaction. Testing in vitro proves that MF4 promotes T,B lymph cells proliferation. Anti-tumour testing in vivo shows that small rat with subcutaneous inoculation is capable of inhibiting Lewis lung cancer and S-180 caruncle's growth, and has promotion functions for NK cell activity and lymph cell transfer of charged Lewis lung cance small rat. The thick shell mussel glycan MF4 is used to prepare new immonological strengthen pharmaceutics and anti-tumour drugs. And it's a new approach for developing Chinese sea biological sources.

Description

technical field [0001] The invention relates to the technical field of medicine, and is a new polysaccharide MF4 with immune-enhancing and anti-tumor activities isolated from the marine animal mussel thick shell. Background technique [0002] Thick-shelled mussel (Mytilus Coruscus) belongs to the Mytilidae family (Mytilidae). It is a warm-water species. In my country, it is mainly distributed in Dalian, Xiaochangshan, Liaoning Province; Qingdao, Tuoji Island, Shandong Province. Daqin Island, Penglai, Yantai, Weihai; Shengsi Islands, Zhoushan Islands, Zhujiajian, Yushan Islands, Xiadachen, Nanji Island in Zhejiang Province; Pingtan, Xiamen, Dongshan in Fujian Province. The shell is large, thick, and wedge-shaped; the adult body is about 100-200mm long, and the shell length is or more than twice the height, and about 2.5-3 times the width. The front end of the shell is tapered, and the back end is wide and round; the shell surface is rough, covered wi...

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Problems solved by technology

The front end of the shell is tapered, and the back end is wide and round; the shell surface is rough, covered with brown or chestnut brown shell skin (Wang Zhenrui, Chinese Zoology. Molluscs. Bivalvia: Mytilidae. 1st edition. Beijing .Beijing Science Press, 1997) Mussel meat is rich in nutrients and is an important source of high-grade protein and vitamin D; literature reports (Liu Zhifeng et al., China Marine Medicine, 2001, 20(6): 9) mussels It has the functions of anti-atherosclerosis, lowering blood fat, inhibiting the aggregation of platelets, thereby inhibiting the formation of thrombus, improving the supply of myocardial oxygen and nutrients, protecting experimental myocardial ischemia, improving microcirculation and lowering blood pressure, etc. The active ingredient of shellfish has not been reported