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Biochemical detection liquid for calcium in urine

A technology of biochemical detection and calcium metabolism, applied in biological testing, analysis through chemical reactions of materials, material inspection products, etc., can solve problems that have not been done before, and achieve the effect of strong versatility and social popularization

Inactive Publication Date: 2005-01-26
赵青华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, for the detection of calcium metabolism, there have been no relevant reports on methods and products suitable for non-clinical "folk use"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of urine calcium biochemical detection solution

[0031] Process requirements:

[0032] Mix oxalic acid, ammonium oxalate and glacial acetic acid in a container above 60°C and stir until fully dissolved, then add distilled water to the required amount. .

[0033] The obtained product is a colorless transparent solution with a pH of 2.25±0.25, and is stored in a white bottle sealed away from light at room temperature.

Embodiment 2

[0035] Sensitivity of urine calcium biochemical detection solution

[0036] Get calcium carbonate aqueous solution and make concentration be 0.2%, 0.1%, 0.05%, 0.025%, 0.012%, 0.006%, 0.003% in 7 test tubes with a capacity of 0.5ml, and indicate the concentration.

[0037] Detection concentration range

[0038] The above experiments show that the sensitivity of the urine calcium biochemical detection solution can reach about 0.01-0.003%.

Embodiment 3

[0040] Calcium metabolism test

[0041] Add 10 drops of 24-hour (12 hours × 2) total urine volume and shake well urine, add the same amount of urine calcium biochemical detection solution to observe: if there is milky white precipitation, white micro-precipitation, white turbidity, light turbidity, microscopic turbidity , slightly muddy, and traces show the test results of different urinary calcium concentrations of 0.2%, 0.1%, 0.05%, 0.025%, 0.012%, 0.006%, and 0.003%. After testing the test tube, use text paper as the background: the background text is clearly magnified as decreased calcium urine (0.006-0.001%); the background text is blurred but can be read as normal urine calcium (0.025-0.012%); the background text is blurred and cannot be read as Increased urinary calcium (0.2-0.05%).

[0042] According to the above urine calcium chromogenic results are more consistent with the classical urine calcium test results.

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PUM

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Abstract

This is a biochemistry detection liquid of urine calcium comprising 1.5-2w / v percents oxalic acid, 1.5-2w / v percents oxalic acid amine, 3.2- 3.5v / v percents glacial acetic acid and distilled water. This invention offers biochemistry detection liquid and self-checking method of urine calcium for the mass scoutting and checking dynamically. Through the recording curve diagram in accordance with series detecting color result one can self-judge something: 1, warning signal of health and pathogenic factor; 2, forepart detectable rate of calcium metabolism overbalancing; 3, the synchronous tracking monitoring of curative effect during the treating course. The above liquid offers the likelihood of carrying out all this function.

Description

technical field [0001] The present invention relates to a urine calcium biochemical detection solution mainly based on urine calcium and its application Background technique [0002] 1 kilogram of the total amount of calcium in the human body accounts for 1.5% of the body weight, and 99% of it is in bone calcium. When the X-ray finds that the bone density has decreased, more than 30% of the calcium has been decalcified. [0003] About 10 grams of calcium is contained in human body fluid (1% is free in blood and extracellular fluid), and plasma calcium is less than 0.3 gram, and serum total calcium concentration is 9-11mg%, accounts for 35% of human body total calcium. [0004] Serum calcium has three forms of calcium: 1. Bound calcium 2. Unbound calcium 3. Ionized calcium. Ionized calcium has a direct physiological effect and participates in physiological regulation reactions, accounting for 53% of the total calcium. [0005] Physiological calci...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/52
Inventor 赵青华
Owner 赵青华
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