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Technique for extracting triterpene substane of mycelium of ganoderma lucidum

A technology of ganoderma lucidum mycelium and extraction process, which is applied in fermentation and other directions, can solve problems such as the impact of industrialization prospects, and achieve the effect of improving safety

Inactive Publication Date: 2005-01-12
徐志祥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Triterpenes are important active ingredients in Ganoderma lucidum. The current academic research mainly focuses on the extraction and pharmacological effects of triterpenes from fruiting bodies or spores. There are few reports on the extraction process of triterpenes from mycelia.
The relevant extraction processes reported in the literature mainly use toxic organic solvents such as methanol, chloroform and ether, which affects the industrialization prospects of such processes to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: After mixing 5 ml of 3.5% lysozyme with one gram of wet mycelia of Ganoderma lucidum, they were reacted in a water bath at 32° C. for 3 hours. After filtering to remove impurities, nitrosoguanidine was added at a concentration of 0.4 mg / ml. Mix the protoplasts of Ganoderma lucidum and nitrosoguanidine, shake for 45 minutes, centrifuge, insert into the fermentation medium and culture for about 8 days, carry out shaking flask culture and screening, and select the strains with high fermentation yield and triterpenoid content as the production strains kind. After preliminary screening and fine screening, a shake flask fermentation yield reached 1.8% and the content of triterpenes was 5.6%. The ganoderma lucidum production strain cultivated in the shake flask was inserted into 500L first-class seedlings, and the composition and proportion of the fermentation medium were: bran 2%, peptone 1%, glucose 0.3%, sucrose 2%, magnesium sulfate 0.05%, Potassium dihydrogen...

Embodiment 2

[0019] Example 2: After mixing 5 milliliters of 3.5% lysozyme with 1 gram of wet mycelia of Ganoderma lucidum, they were reacted in a water bath at 32° C. for 3 hours. After filtering to remove impurities, nitrosoguanidine was added at a concentration of 0.4 mg / ml. Mix the protoplasts of Ganoderma lucidum and nitrosoguanidine, shake for 45 minutes, centrifuge, insert into the fermentation medium and culture for about 8 days, carry out shaking flask culture and screening, and select the strains with high fermentation yield and triterpenoid content as the production strains kind. After preliminary screening and fine screening, a shake flask fermentation yield reached 1.8% and the content of triterpenes was 5.6%. Connect the ganoderma lucidum production strain cultured in the shake flask into a 500L primary seed tank, and the composition and proportion of the fermentation medium are: bran 2.6%, peptone 1.7%, glucose 0.36%, sucrose 1.5%, magnesium sulfate 0.04%, Potassium dihydr...

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Abstract

An extraction technology for industrialized ganoderic mycelia trilerpepe substances is successfully set up by reforming the ganoderic seeds and improving the fermentation technology, parameters, culture media recipes and extraction technology. The reformed seeds contains much more trilerpenes, its fermentation scale is higher than the present and the fermentation technology is favorable to the synthesis and accumulation of ganoderic trilerpene substances, the edible extraction solvent increases safety of production. The content of trilerpepe in said hyphae powder reaches to 5-7g / 100g and extracted content is more than 25%.

Description

technical field [0001] The invention relates to an industrial extraction process of ganoderma lucidum mycelium triterpenes. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum (Fr.) Karst] is also known as Ruicao and Ganoderma lucidum, and its English name is Reishi mushroom. According to the fungal classification system of Ainsworth1973, Ganoderma lucidum belongs to Basidiomycotina, Hymenomycetes ), Holobasidiomycetidae, Aphyllophorales or Polyporales, Ganodermataceae, Ganoderma. [0003] Ganoderma lucidum has a long history of medicinal use in my country. According to ancient books, the understanding and utilization of Ganoderma lucidum in my country should have begun in Yao (2100 BC), which has a history of at least 4,000 years. As early as "Lie Zi Pian" (1100 B.C.), there were "records of fungus mushrooms on the rotten soil", and the earliest pharmacy monograph in my country, "Shen Nong's Herbal Classic" written in the Eastern ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00
Inventor 徐志祥
Owner 徐志祥
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