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Method for preparing multifunctional biological repair material

A bioremediation and multifunctional technology, applied in the field of biomedicine, achieves the effects of low price, adjustable degradation speed and convenient use

Inactive Publication Date: 2004-10-27
刘永庆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan, known as nerve repair, still has some problems in its mechanical strength, degradation speed and repair effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Dissolve chitosan, type I collagen, and sodium hyaluronate with a deacetylation degree of 50% and a molecular weight of 400,000 Daltons in 1% acetic acid solution or distilled water to make a shell with a concentration of 2%. Polysaccharide acetic acid solution, 1% type I collagen acetic acid solution and 1% sodium hyaluronate aqueous solution; adjust the pH of sodium hyaluronate aqueous solution to about 3 with 1M HCI, then press 1:50:200 (v / v) Mix the sodium hyaluronate solution with the collagen and chitosan solutions evenly.

[0019] (2) lyophilize the prepared solution into a container to form a sponge layer film with a thickness of 3 mm or an internal diameter of 2 mm and a porous tubular material with an external diameter of 5 mm; (note: the tubular material is made of chitosan / collagen / Sodium hyaluronate solution is injected into a ring-shaped container made of concentric tubular polytetrafluoroethylene, then quickly frozen and dried.)

[0020](3) After i...

Embodiment 2

[0023] (1) Sodium hyaluronate, type II collagen, chitosan with a deacetylation degree of 70% and a molecular weight of 200,000 Daltons were dissolved in distilled water or 1% acetic acid solution respectively to make a transparent gel with a concentration of 10%. Sodium hyaluronate aqueous solution, 2% type I collagen, 1% chitosan acetic acid solution; use 1M HCI to adjust the pH of sodium hyaluronate aqueous solution to about 2, and then press 1:20:50 (v / v) Mix the sodium hyaluronate solution with the collagen and chitosan solutions evenly.

[0024] (2) freeze-dry the prepared solution in a container to form a sponge with a thickness of 4 mm or a porous tubular material with an inner diameter of 3 mm, an outer diameter of 5 mm, and a length of 15 mm;

[0025] (3) Soak the sample with 40% ammonia water for 10 minutes; then rinse it with distilled water, and freeze-dry it.

[0026] (4) Apply a thin layer of 2% chitosan solution (dry until it will not dry) on the polytetrafluor...

Embodiment 3

[0029] (1) Sodium hyaluronate, type IV collagen, chitosan with a deacetylation degree of 90% and a molecular weight of 10,000 Daltons were dissolved in distilled water or 2% acetic acid solution respectively to make a concentration of 3% chitosan Sugar solution, 0.1% sodium hyaluronate aqueous solution, 2% type I collagen, 2% chitosan acetic acid solution; adjust the pH of sodium hyaluronate aqueous solution to about 1 with 1M HCI, and then press 1:1 : 1 (v / v) ratio Mix sodium hyaluronate solution with collagen and chitosan solution evenly.

[0030] (2) freeze-dry the prepared solution in a container to form a sponge with a thickness of 0.5 mm or a porous tubular material with an inner diameter of 2 mm and an outer diameter of 3 mm;

[0031] (3) The sample was soaked in 25% ammonia water for 20 minutes; then rinsed with distilled water, and then freeze-dried.

[0032] (4) When the solution prepared in step (1) is baked until it is almost dry, add a small amount of sodium chlo...

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Abstract

A multifunctional biologic material for repairing skin, nerve and cartilage is prepared from chitosan, collagen and sodium hyaluronate through preparing their solutions, proportionally mixing, defomaing, loading in mould, freeze drying to become film or tube, immersing in ammonia water, drying, coating a solution layer of chitosan, chitosan / sodium hyaluronate, chitosan / collagen, or chitosan / collagen / sodium hyaluronate, and drying. Its advantages are high biocompatibility, mechanical strength and antibacterial power.

Description

technical field [0001] The invention relates to a preparation method of biological materials used for skin, nerve and cartilage repair, which mainly uses chitosan / collagen / sodium hyaluronate as raw materials to prepare thin film or tubular materials, and belongs to the technical field of biomedicine. Background technique [0002] The skin and nervous system are the most important tissues and organs in the human body. Once damaged, their normal functions will be affected. Although the regeneration ability of the skin is very strong, it is far from enough to heal large area burns or wounds by self-skin or autograft skin alone, and it is necessary (artificial skin as) a temporary wound surface during the treatment process Protective covering materials come to help. Artificial skin made of synthetic materials or collagen has been used clinically for many years, but there are certain defects. Chitosan is the only basic polysaccharide among the known natural polysaccharides. Its...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/60
Inventor 刘永庆
Owner 刘永庆
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