Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

N-end fusion protein PC-1-e2 of human multicapsular protein-1

A polycystin and fusion protein technology, applied in the field of medical molecular bioengineering, can solve the problems of high cost, short half-life, and limited intervention effect, and achieve low cost and long-lasting inhibitory effect

Inactive Publication Date: 2004-09-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Intervention measures to inhibit abnormal proliferation of renal tubular epithelial cells have been reported, such as lovastatin, tyrosine protein kinase inhibitors, etc., which are not only expensive, but also have limited intervention effects, and because the involved signal transduction pathways are complex and have a short half-life and other reasons, resulting in many uncontrollable factors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • N-end fusion protein PC-1-e2 of human multicapsular protein-1
  • N-end fusion protein PC-1-e2 of human multicapsular protein-1
  • N-end fusion protein PC-1-e2 of human multicapsular protein-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The preparation of human PC-1 N-terminal fusion protein PC-1-e2 is now described in detail.

[0026] 1. Materials

[0027] 1. Plasmids and strains: fusion protein expression vector pQE30 (the 5' end of its reading frame contains a nucleotide sequence encoding 6 consecutive histidines, and the plasmid contains an ampicillin resistance marker gene) and expression host bacterium M15 (the strain A repressor plasmid pREP4, which contains a kanamycin resistance marker gene) was purchased from Qiagen, Germany.

[0028] 2. Main reagents and instruments The total RNA extraction kit and gel recovery kit are products of Huashun Company, and the One-Step RT-PCR kit and Ni-NTA purification reagent (Ni-NTA Agarose) were purchased from Qiagen, Germany. Various restriction enzymes, T 4 DNA ligase is a product of Takara Company. Ampicillin (Amp) and Kanamycin (Kan) were purchased from Shanghai Huamei Bioengineering Company. Fetal bovine serum, RPMI 1640 and DMEM cell culture medium ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to medicine molecular biology engineering technology, and is one kind of 1N-end fusion protein PC-1-e2 of human polycystic protein-1. According to cDNA sequence of human polycystic kidney disease-1 gene and the molecular structure of its encoded product, polycystic protein-1 (PC-1), the gene is cloned to cDNA-PKD1e2 in the N end of the protein and its prokaryotic expression carrier pQE30-PKD1e2 is constituted by means of molecular biology engineering technology. N-end fusion protein PC-1-e2 of PC-1 is induced in colibacillus. Cytobiology experiment shows that the fusion protein has obvious inhibition to the proliferation of the epidermis cell line of in vitro cultured human polycystic kidney cyst lining and the epidermis cell strain of far end renal tubule in dog kidney, so that it may be used in preparing medicine for treating autosomal dominant hereditary polycystic kidney disease, the reagent for the mechanism research of the disease, and anti-PC-1 antibody.

Description

technical field [0001] The invention relates to the technical field of medical molecular bioengineering, which is a human polycystin-1 N-terminal fusion protein PC-1-e2, which can be used to prepare medicine for treating autosomal dominant polycystic kidney disease, and can be used to prepare and study the Reagents of disease pathogenesis and preparation of anti-PC-1 antibodies. Background technique [0002] Autosomal dominant polycystic kidney disease (ADPKD) is a hereditary kidney disease that seriously endangers human health. Patients with bilateral kidneys are densely covered with cysts, and often develop into end-stage renal failure in late middle age. Due to its The pathogenic gene is widely expressed in the body, and often involves other epithelial organs, including the liver, pancreas, ovary and choroid plexus, etc., causing great harm. It is now clear that 85-90% of the occurrence of ADPKD is due to the structural and functional abnormal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/17A61P13/12C07K16/18C07K19/00G01N33/68
Inventor 赵海丹梅长林
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com