Use of CoCl#-[2] in preparation of drug for treating leukemia
A technology for leukemia and leukemia cells, used in drug combinations, anti-tumor drugs, pill delivery, etc.
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Embodiment 3
[0135] In order to understand HIF-1α and CoCl 2 Whether there is a connection between the differentiation of induced leukemia cells, the present invention uses semi-quantitative RT-PCR technology and Western blot technology to detect CoCl 2 Possible effects on the expression levels of HIF-1α mRNA and protein in cells ( Figure 10 ). The results showed that 50 μM CoCl 2 Treatment did not alter HIF-1α mRNA levels in all three AML cell lines examined, but it rapidly increased HIF-1α protein levels in NB4 and U937 cells. Therefore, like most organizations, CoCl 2 It is beneficial for these two kinds of cells to stabilize the HIF-1α protein level. It is worth mentioning that HL60 cells also express HIF-1α and normal HIF-1α cDNA sequence at the mRNA level, but CoCl 2 However, it did not induce the stable expression of HIF-1α protein in HL60 cells.
[0136] 10. Inhibition of HIF-1α protein level will significantly antagonize CoCl 2 Differentiation-inducing effect ---
Embodiment 4
[0138] In order to further clarify whether HIF-1α is involved in CoCl 2 Inducing the differentiation process of leukemia cells, using the inhibitor of this protein to observe its influence on cell induction effect is a common method in current biological research. According to literature reports, certain nitric oxide donors, such as 3-morpholinosychnonimine (SIN-1), can reduce the stability of HIF-1α expression. Therefore, we observed that under the premise of reduced expression of HIF-1α protein, its effect on CoCl 2 The effect of inducing differentiation effect ( Figure 11 ). The results showed that 500 μM SIN-1 almost completely inhibited the aggregation of HIF-1α in NB4 and U937 cells. Meanwhile, SIN-1 effectively antagonized CoCl while inhibiting the growth of NB4 and U937 cells 2 Therefore, it can be seen that HIF-1α mediates or participates in the process of hypoxia-induced leukemia cell differentiation.
[0139] Through the aforementioned experiments and analysis...
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