Use of CoCl#-[2] in preparation of drug for treating leukemia
A technology of leukemia and medicine, applied in the field of application of CoCl2 in the preparation of medicines for treating leukemia
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Embodiment 3
[0130] To understand HIF-1α and CoCl 2 Whether the differentiation of induced leukemia cells is related, the present invention uses semi-quantitative RT-PCR technology and Western blot technology to detect CoCl 2 Possible effects on the expression levels of HIF-1α mRNA and protein in cells ( Picture 10 ). The results show that 50μM CoCl 2 The treatment did not change the HIF-1α mRNA levels of all three AML cell lines tested, but it could rapidly increase the HIF-1α protein levels in NB4 and U937 cells. Therefore, like most organizations, CoCl 2 It is helpful for these two cells to stabilize the HIF-1α protein level. It is worth mentioning that HL60 cells also have the expression of HIF-1α at the mRNA level and the normal HIF-1α cDNA sequence, but CoCl 2 It does not induce stable expression of HIF-1α protein in HL60 cells.
[0131] 10. Inhibition of HIF-1α protein level will significantly antagonize CoCl 2 Inducing differentiation effect---
Embodiment 4
[0133] To further clarify whether HIF-1α is involved in CoCl 2 In the differentiation process of induced leukemia cells, the use of inhibitors of this protein to observe its effect on cell induction is a common method in current biological research. According to reports in the literature, certain nitric oxide donors, such as 3-morpholinosychnonimine (SIN-1), can reduce the stability of HIF-1α expression. Therefore, we observed that under the premise of reduced expression of HIF-1α protein, its effect on CoCl 2 The influence of induced differentiation effect ( Picture 11 ). The results showed that 500μM SIN-1 almost completely inhibited the accumulation of HIF-1α in NB4 and U937 cells. At the same time, SIN-1 can effectively antagonize CoCl while inhibiting the growth of NB4 and U937 cells. 2 The induced cell differentiation, therefore, it can be known that HIF-1α mediates or participates in the differentiation process of leukemia cells induced by hypoxia.
[0134] Through the for...
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