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Cordyceps vegetative stage solid fermentation technology

A technology of solid fermentation and solid fermentation tank, applied in the direction of fungi, etc., can solve the problems of complex process, large energy consumption, large equipment investment, and large loss of functional components, and achieve the effects of simple post-processing, quality improvement, and cost saving

Inactive Publication Date: 2004-07-21
张雪峰
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Problems solved by technology

[0008] The technical problem to be solved by the present invention is: to overcome the defects of large loss of functional components of the liquid fermentation mycelium of Cordyceps sinensis in the asexual stage, low yield, complex process, numerous procedures, easy pollution, energy consumption and large investment in equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] Cordyceps sinensis strain: Hirsutella Sinensis Liu, Guo, Yu et Zeng. with strain preservation number CCTCC No. M87101.

[0036] 1. Incline cultivation:

[0037] The medium is: bran 30g, peptone 10g, milk powder 10g, silkworm chrysalis powder 3g, vitamin 10mg, magnesium sulfate 0.5g, potassium dihydrogen phosphate 1.0g, agar 20g, H2O 1000ml, pH6.5.

[0038] The Mortierella sinensis strain is inoculated on a slant medium and cultured at 15-18°C for 15-40 days.

[0039] 2. Liquid culture

[0040] Liquid medium: bran 30g, milk powder 10g, silkworm chrysalis powder juice 3g, beef juice 10g, vitamin 10mg, magnesium sulfate 0.5g, potassium dihydrogen phosphate 1.0g, water 1000ml. Culture solution pH: 6.5.

[0041] The culture medium is divided into Erlenmeyer flasks, the volume is about 1 / 3 of the Erlenmeyer flasks, sterilized and placed in a shaker, at a temperature of 18±2°C, 100-150 times / min shaking on a shaker for 120-160 hours , and then expand the culture.

[0042]...

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Abstract

A solid fermenting process of cordyceps features use of Hirsutella sinensis (CCTCC No.M87101) is used as the fermenting bacterial strain, and includes bacterial strain culture, solid fermenting culture, discharging mycelia, drying and pulverizing. Its advantages are high output rate (20-40%), no environmental pollution and low cost.

Description

technical field [0001] The invention relates to a solid fermentation process of the asexual stage of Cordyceps sinensis (Berk.) Sacc., in particular to a solid fermentation process of Hirsutella Sinensis Liu, Guo, Yu et Zeng. technical background [0002] Cordyceps sinensis is a rare fungus Chinese herbal medicine, a species of the genus Cordyceps of the family Ergotaceae, which parasitizes on the larvae of the family Batidae and completes the sexual stage. Because Cordyceps sinensis is a psychrophilic bacterium that parasitizes the larvae of the Cordyceps bat moth that only grows in meadows at altitudes of 3500-5000 meters, it is difficult to cultivate artificially. Since the 1980s, the research on Cordyceps sinensis has gradually increased. In 1984, Mr. Chen Qingtao believed that Paeciomyces sinensis Chen, Xiao et Shi. is the asexual stage of Cordyceps sinensis (Chen Qingtao et al.: 1984, a new species of Paecilomyces sinensis in China Relationship with Cordyceps. Acta My...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 俞永信
Owner 张雪峰
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