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Method for preparing young pine infected by armillaria matsutake

一种松树、蘑菌的技术，应用在植物学设备和方法、园艺方法、真菌类等方向，能够解决复杂实验技术、没有高产量等问题

Inactive Publication Date: 2004-05-12
庆尚北道知事
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the identification of growing fungi requires sophisticated experimental techniques
Therefore, pine trees infected with Matsutake fungus were not obtained in high yields

Method used

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  • Method for preparing young pine infected by armillaria matsutake
  • Method for preparing young pine infected by armillaria matsutake
  • Method for preparing young pine infected by armillaria matsutake

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of young pine trees infected with matsutake mushrooms using a culture vessel for Flammulina velutipes

[0045] In order to obtain such Figure 4 Young pine trees infected with Matsutake mushroom shown, sterilized K-solid medium, which is usually used for cultivating Flammulina velutipes mushrooms, was poured into a sterilized culture vessel and allowed to harden . After covering the K-solid medium with filter paper, the mycelium and 5ml sterile water were inoculated on the filter paper with a dry weight of 0.075mg. A mixture of perlite and sphagnum peat moss was added to the inoculated fungal mycelia and K-broth pH 5.6 was added to the soil to form an infection medium. Then, the sprouts obtained from germinating pine sub-germinations under aseptic conditions were planted on the infection medium containing mixed soil and K-liquid medium, and the culture container was covered with a cover, and then the pine trees were grown at 20° C. under the ligh...

preparation example 1

[0049] Preparation Example 1: Preparation of Tricholoma matsutake inoculum from liquid culture

[0050] The matsutake fungus used as an inoculum in the present invention was isolated from a naturally occurring matsutake mushroom just before its cap opened from Gyeongju, Gyeongsangbuk-do, Korea. -si) Collected from 10 hectares (ha) of Doyoo Forest in Namsan-dong. Within 8 hours after collection, the part between the cap and the gills in the collected mushrooms was cut into 0.5 mm sized pieces, and planted on the MMN medium of pH 5.5 according to the composition shown in Table 2 below. Then, the isolated mycelia were further cultured on PDA medium for 60 days at the optimum growth temperature for matsutake mushroom growth, 23±0.5°C. The matsutake fungus was successfully isolated from about 98% of the cultured mycelia. It was found that the isolated mycelia had more than 99% homology with the known ITS sequence, the entire 5.8S rRNA and part of the 18S DNA sequence. The identi...

preparation example 2

[0053] Preparation Example 2: Preparation of Infection Medium Using Mixed Soil and K-Liquid Medium

[0054] Perlite and peat moss peat bog were used as bed soil for cultivating pine sprouts. After mixing in a ratio of 80:1.5, they were sterilized in containers. On the ultra-clean bench, pour the sterilized mixed soil into the culture container inoculated with the matsutake mycelium to a suitable height.

[0055] K-liquid medium was prepared according to the composition shown in Table 1 above. After autoclaving at high temperature, pour 100 ml of K-liquid medium onto the mixed soil on a clean bench.

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Abstract

Disclosed is a method of preparing Tricholoma matsutake-infected young pine trees by coculturing aseptic seedlings of a pine tree with a T. matsutake isolate, comprising the steps of liquid-culturing fruit bodies of naturally occurring matsutake mushrooms in PDB (Potatto Dextrose Broth, Difco) and inoculating T. matsutake KBFERI 20T05 isolated from the cultured fruit bodies; placing mixed soil of perlite and sphagnum peatmoss and K-liquid medium onto the inoculated fungus; and planting aseptic seedlings obtained by aseptically germinating pine seeds into the mixed soil, and then culturing the pine seedlings with the T. matsutake isolate to allow ectomycorrhiza formation on the fine roots of the pine seedlings. The method of the present invention is advantageous in terms of giving objectivity for the fungal inoculum by employing as an inoculum a fungus having DNA homology with the conventionally known T. matsutake, and of making it possible to mass produce T. matsutake-infected pine trees throughout the Year.

Description

technical field [0001] The invention relates to a method for preparing young pine trees infected with Tricholoma matsutake by cocultivating aseptic pine tree sprouts and Tricholoma matsutake. More specifically, the present invention relates to a method for selectively infecting the roots of pine shoots only with Matsutrimon by culturing the aseptic pine shoots together with the mycelia of Matsuzuki isolates. Background technique [0002] Pine mushroom is a fungus belonging to the subclass Basidiomycotina (Basidiomycotina), Agaricales (Agaricales) and the family Tricholomataceae (Tricholomataceae), which naturally grows in including Pinus densiflora, Pinus pumila, European spruce (Piceaabies) ) and Picea jezoensis coniferous forests. In particular, in Korea, the fungus is known to grow only in pine forests. In Korea and Japan, matsutake mushroom is one of the most popular edible mushrooms, especially in the eastern seaboard of Korea, where it is the main economic source. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01G23/00A01H4/00A01H5/00A01H17/00C12N1/14
CPCA01G1/048A01G1/04A01G18/10A01G18/20A01G18/50A01G18/64A01H4/001A01H4/005
Inventor 朴武昌沈相甲千于宰
Owner 庆尚北道知事
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