Process for preparing chicken colibacillus pilus monoclonal antibody

Abstract

A method of preparing monoclonal antibody against pillis of bacillus coli. It comprises: 1) screening good strains of bacillus coli; 2) preparing immunogens;3) immunizing BALB / C mice;4)fusing hybridoma cells; 5) screening hybridoma cells; 6) screening positive hybridoma cells;7) clonizing;8) clonizing again;9) inducing ascitic fluid containing monoclonal antibody; 10) measuring valence of monoclonal antibody in ascitic fluid. The monoclonal antibody has a high valence, could discriminate F1,P and other pillis, which could be used to determine pathogenic bacillus coli strains.

Description

technical field [0001] The invention belongs to the technical field of biological cell engineering, and in particular relates to a preparation method of chicken Escherichia coli pili monoclonal antibody. Background technique [0002] Chicken colibacillosis is a contact infectious disease caused by pathogenic Escherichia coli, and it is one of the most serious infectious diseases that endanger the chicken industry. There are more than 170 serotypes of chicken Escherichia coli, but most of them are non-pathogenic, and only a few are pathogenic bacteria. Its pili play an important role in the process of pathogenic Escherichia coli invading the body, colonizing the body, proliferating, and causing disease. There are many types of Escherichia coli pili and they are complex. But for chicken pathogenic Escherichia coli, only two important pili, F1 and P, have been found so far. Both pili are coded by the chromosome of the bacterium, and their molecular wei...

AI Technical Summary

Problems solved by technology

At present, there are many researches on F1 and P pili at home and abroad, but the research is greatly limited.

The main reason is that the pili antiserum used in current research is polyclonal antiserum, which has poor specificity, cross-reactivity, low titer, large batch-to-batch variation, troublesome preparation, difficult promotion, difficult standardization, and inability to effectively distinguish F1, P and other fimbriae cannot meet the needs of research

In addition, the prevention and treatment of colibacillosis mainly depends on vaccines, but the current E. coli vaccines generally reflect unsatisfactory effects. The main reason is that it is impossible to screen excellent vaccine-making strains, and it is also impossible to determine their pathogenicity and the expression of F1 and P pili.

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