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Composite fluorescent nano particle used for immunoassay and its preparation method

An immunological detection and nanoparticle technology, which is applied in the direction of measuring devices, scientific instruments, biological testing, etc., can solve the problems of difficult simultaneous detection of multiple indicators, low resolution, poor photostability, etc., and achieve excellent chemical stability and The effect of biocompatibility

Inactive Publication Date: 2003-12-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, organic fluorescent dye labeling has the advantages of high luminous intensity and good detection sensitivity, but generally the fluorescence peaks of organic molecules are relatively broad, and the resolution in multi-component detection is not high, and organic molecules with different luminescence generally have different excitation wavelengths, so that It is difficult to achieve simultaneous detection of multiple indicators under single-wavelength excitation
In addition, the light stability of organic dye molecules is poor, and photobleaching or photodegradation will occur when the excitation light intensity is high or the time is long.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Get 5 mg of CdS nanoparticles with a particle diameter of 1.9 nm, dissolve them in 120 ml of methanol containing 0.17% triethoxymercaptopropylsilane, 25% dimethyl sulfoxide, and use (CH 3 ) 4 NOH·5H 2 O to adjust the pH value between 10-11, after stirring the reaction for 12 hours, add 40ml10% trimethylchlorosilane solution, use (CH 3 ) 4 NOH·5H 2 O adjust the pH value between 10-11, stir the reaction for 12 hours, then concentrate in vacuo at 60°C to an oily liquid, add acetone:isopropanol=1:1 mixed solvent to precipitate CdS. After the precipitate was washed twice with acetone, 50ml of ethanol was added for ultrasonic redispersion, 2ml of concentrated ammonia water with a concentration of 23%, 10μL of ethyl orthosilicate, and 2μL of triethoxyaminopropylsilane were added, and the reaction was stirred for 6 hours to obtain coating SiO 2 The luminescent particle sol with a layer thickness of 10nm was centrifuged at a speed of 5000rpm for 60 minutes, and the supernat...

Embodiment 2

[0016] Take 6 mg of CdS nanoparticles with a particle size of 2.4 nm as the luminescent core, and perform surface modification according to the steps in Example 1 to obtain a sol ultrasonically dispersed in ethanol, then add 2 ml of 23% concentrated ammonia, 20 μL of ethyl orthosilicate, 4 μL of triethoxyaminopropylsilane was stirred for 8 hours to obtain coated SiO 2 The luminescent particle sol with a layer thickness of 20 nm was subjected to gold deposition according to the reaction conditions in Example 1. Composite luminescent particles with a luminescence peak of 680 nm were finally obtained.

Embodiment 3

[0018] Take 10 mg of CdSe nanoparticles with a particle size of 3.6 nm as the luminescent core, and perform surface modification according to the steps in Example 1 to obtain a sol ultrasonically dispersed in ethanol, then add 2 ml of concentrated ammonia water with a concentration of 23%, and 25 μL of ethyl orthosilicate , 4 μL triethoxyaminopropyl silane, stirred for 8 hours to obtain coated SiO 2 For the luminescent particle sol with a layer thickness of 25nm, transfer the particles to the water phase according to the method in Example 1, add 3ml of the sol containing 2nm gold particles (1×10-3mol / L), stir and react for 2 hours, and obtain the thickness of gold deposited on the surface The core of 7nm is a composite luminescent particle of CdSe, and the luminescence peak of this composite particle is at 680nm.

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Abstract

A composite fluorescent nanoparticles for immunodetection is composed of the semiconductor nanoparticle as kernel, such as CdS, CdSe, or ZnS, SiO2 layer coated on the surface of said kernel, and island-shaped gold layer deposited on the SiO2 layer. Its preparing process is also disclosed. Its advantages are high stability and high resolution.

Description

Technical field: [0001] The invention relates to a composite fluorescent nanoparticle for immunological detection and a preparation method thereof. Background technique: [0002] Biological detection, especially immunological detection, has become an important method and basis for clinical diagnosis and treatment. In principle, high-sensitivity immune detection can realize the early diagnosis of major diseases, especially cancer, which is undoubtedly of great significance to human health. [0003] At present, the clinically used immunoassay technology mainly uses probe labeling methods, such as radioactive labeling, enzyme labeling, and organic fluorescent dye labeling. Among them, organic fluorescent dye labeling has the advantages of high luminous intensity and good detection sensitivity, but generally the fluorescence peaks of organic molecules are relatively broad, and the resolution in multi-component detection is not high, and organic molecules with different luminesc...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/533
Inventor 庄家骐杨文胜张晓冬白玉白李铁津
Owner JILIN UNIV
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