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New pharmaceutical of cactus total flavone and its preparation method

A technology of cactus and total flavonoids is applied in the field of medicine to achieve the effects of stable production process, easy realization and exact curative effect

Inactive Publication Date: 2003-09-17
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports about the inhibitory effect of cactus plant extracts on aldose reductase and the application of treatment and health care in diabetic complications

Method used

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  • New pharmaceutical of cactus total flavone and its preparation method
  • New pharmaceutical of cactus total flavone and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation method example of cactus total flavonoids

[0024] 1. Preparation of total flavonoids from cactus by solvent extraction / solvent extraction

[0025] 1.5 kg of dried cactus coarse grains are extracted by 80% ethanol (15L×3) under reflux at 90°C, the extracts are combined (concentrated to obtain 133 grams of extract), the ethanol extract is recovered from the ethanol solution, concentrated to a small volume, Extracted three times with water-saturated equal amount of n-butanol, combined the extracts, concentrated under reduced pressure to recover n-butanol, then extracted three times with water-saturated equal amount of ethyl acetate, recovered the solvent under reduced pressure to dryness, and obtained The yield of total flavonoids is about 0.9%.

[0026] 2. Preparation of total flavonoids from cactus by solvent extraction and polyamide column chromatography

[0027] 1.5 kg of dried cactus coarse grains are extracted by 80% ethanol (15L×3) u...

Embodiment 2

[0030] Embodiment 2: the inventor has done following experimental research in order to prove the active effect of inhibiting aldose reductase of the present invention in the development process:

[0031] 1. Inhibition of aldose reductase activity by cactus extract

[0032] (1) Preparation of aldose-reduced aldehyde in rat lens

[0033] Wistar rats were sacrificed, their lenses were taken out, put into 135mM Na, K-phosphate buffer (PH7.0) containing 0.5mM phenylmethylsulfonyl fluoride (PMSF) and 10mM 2-mercaptoethanol, and homogenized , centrifuge at 100,000×g for 30 minutes, take the supernatant, and store it at 4°C.

[0034] (2) Experiment on the activity of cactus extract in inhibiting aldose reductase

[0035] Take the above rat lens aldose reductase, dilute 10-40 times with 180mM Na, K-phosphate buffer (PH7.0), take 100μl, add 135mM Na, K-phosphate buffer (PH7.0) 225μl, 100mM Li2S04 50μl, 0.03mM NADPH constitute the culture medium, 100μl of 1mM DL-glyceraldehyde is used...

Embodiment 3

[0038] Embodiment 3: Example of the preparation method of the present invention for treating diabetic complications medicine or health food.

[0039] 1. Mix 10 grams of cactus total flavonoids with 40 grams of cornstarch and add water to make a soft material, pass through a 12-mesh sieve to granulate, and dry to obtain granules. In this granule, every 500mg contains 100mg of total flavonoid extract. Combined with symptoms, 0.5-1 gram each time, 3-4 times a day.

[0040] 2. Mix 80 grams of total flavonoids of cactus with 110 grams of lactose and 10 grams of magnesium stearate, and fill capsules with 600 mg of each. In this capsule, each capsule contains 150 grams of total flavonoids of cactus. Combined with symptoms, 2-3 times each time, 3-4 times a day.

[0041] 3. 50 grams of cactus total flavonoids are mixed with 45 grams of microcrystalline cellulose and 5 grams of magnesium stearate, and the mixture is punched into tablets with a diameter of 6 mm and a weight of 300 mg w...

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Abstract

A medical application of general cactus flavone in preparing the natural medicine or health-care food acting to suppress aldose reductase for preventing and treating the complication of diabetes suchas the healing defect of corneal injury, cataract, neuropathy, retinopathy and renal disfunction. The said general cactus flavone is prepared by the solvent extraction method or resin adsorption method and conventional drying method.

Description

Technical field: [0001] The invention relates to the technical field of medicine, in particular it is a new medical application of cactus total flavonoids and a preparation method thereof. Background technique: [0002] Diabetes has become a common and frequently-occurring disease that threatens human health. According to the fifth International Diabetes Federation Western Pacific Conference held in Beijing, there are currently 130 million people with type II diabetes diagnosed worldwide, and more than 40 million people in my country; type II diabetes will be developed in China, India and other developing countries in the 21st century. It is prevalent in the country. By 2005, the number of diabetic patients in the world will exceed 300 million. The total number of diabetic patients in my country is close to 100 million, becoming the second largest country in the world in terms of the number of diabetic patients after India. In 1994, the medical expenses for diabetes and card...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P3/10A61P13/12A61P27/02A61P27/12
Inventor 陈英杰邱鹰昆付文卫裴玉萍窦德强徐绥绪
Owner SHENYANG PHARMA UNIVERSITY
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