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Tissue engineered bone tissue

A technology of tissue engineering and bone tissue, applied in the field of biomedical engineering, can solve the problems of lack of bone mineral, lack of basic mechanical strength, lack of basic mechanical stiffness, etc., and achieve good biocompatibility

Inactive Publication Date: 2003-04-16
谭强 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former does not contain organic components, so it is loose and brittle, and does not have basic mechanical strength; the latter lacks bone mineral, so its texture is soft and does not have basic mechanical rigidity

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1) In vitro isolation and culture of bone marrow mesenchymal stem cells:

[0026] Extract 5-10ml of the patient's autologous bone marrow, separate it with Ficoll, extract nucleated cells, and wash twice with PBS+EDTA. Add DMEM (purchased from GIBCOBRL Company. Contains 20% fetal bovine serum) culture medium, place on a fibronectin (Fbronectin)-coated culture dish, and incubator at 37°C containing 5% carbon dioxide. After two days, unattached cells were removed and cultured in conditioned medium.

[0027] Subculture When the cells are confluent to 60%-75% density, rinse with 10ml calcium and magnesium-free PBS, digest with 0.25% trypsin at 37°C for 5 minutes, add DMEM culture solution containing 10% fetal bovine serum to stop digestion, and transfer to a centrifuge tube Centrifuge at 1200r / min for 5 minutes. Discard the supernatant, add 10 ml of DMEM culture solution containing 10% fetal bovine serum, mix well, count, and subculture at a ratio of 1:3. Change the cultu...

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Abstract

A histo-engineered bone tissue is composed of osteocortex frame as externally layer and central loose bone inoculated by bone marrow stem cells. It can be moulding-shape according to the actual operation scope predefined by CT film and CT reconstitution data of deficit part.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a tissue-engineered bone tissue and a preparation method thereof. Background technique [0002] Tissue engineering is a hot spot in life science in the 21st century. Its core content is three-dimensional cell culture in vitro, and its basic method is to form a complex of seed cells and biodegradable biomaterials to replace damaged tissues and organs. At present, autologous bone is mainly used as bone tissue substitutes at home and abroad, accounting for about 70%. Relatively successful in xenograft bone are some bone mineral materials that have completely removed the organic components of bone, such as KEIL bone and BIOSOSS bone. And inorganic bone, and organic component materials that completely remove bone mineral components, such as demineralized bone, bone matrix gelatin, etc. The former does not contain organic components, so it is loose and brittle, and ...

Claims

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Application Information

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IPC IPC(8): A61B17/56A61F2/28A61L27/00
Inventor 谭强赵毅
Owner 谭强
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