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Probe medium and its mfg. process

A probe and medium technology, which is used in the field of probe-fixing substrates

Inactive Publication Date: 2007-03-07
CANON KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in terms of probe extraction, in order to bind probes represented by nucleic acids such as DNA and RNA to nucleic acid-binding carriers, it is known to use a carrier suspended in a solution containing a surfactant to bind to the nucleic acid. to extract it; however, dissolving the probe in a solution containing a surfactant is unknown for use as a probe medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] (1) Synthesis of probes

[0062] A single-stranded DNA probe is used as a probe capable of specifically binding to a target substance. The single-stranded DNA probe of SEQ ID NO: 1 was synthesized using an automatic DNA synthesizer (manufactured by Applied Biosystems, Model 1380A). It should be noted that an 18-fold oligomer prepared at the end of the single-stranded DNA of SEQ ID NO: 1, in which the hydroxyl group at the 5' end was bonded to the amino group via a phosphate group and a hexamethylene group, was used for the following experiments.

[0063] Serial number: 1

[0064] 5'H 2 N-(CH 2 ) 6 -PO 2 -O-ACTGGCCGTCGTTTTACA 3’

[0065] (2) Probe solvent

[0066] Water was used as the probe soluble medium.

[0067] (3) Probe soluble substances

[0068] Cetyltrimethylammonium bromide (n-hexadecyltrimethylammonium bromide) was used as a substance for dissolving the probe in an organic solvent. In order to obtain an aqueous solution in which cetyltrimethylammoniu...

Embodiment 2

[0085] (1) Synthesis of probes

[0086] After preparing the single-stranded DNA probe exactly as in Example 1, it was used in the following experiments.

[0087] (2) Probe solvent

[0088] In exactly the same manner as in Example 1, water was used as a probe-soluble solvent.

[0089] (3) Probe soluble substances

[0090] Cetyltrimethylammonium chloride (n-hexadecyltrimethylammonium chloride) was used as a substance for dissolving the probe in an organic solvent. In order to obtain an aqueous solution in which cetyltrimethylammonium chloride was completely dissolved, it was dissolved while heating in a water bath at 50°C.

[0091] (4) Probe insoluble solvent

[0092]An isocyanate silane coupling agent, isopropanol, and dipropylene glycol were prepared as a probe-insoluble organic solvent in exactly the same manner as in Example 1, and were used for the following tests.

[0093] (5) Modulation of probe medium

[0094] A sample obtained by dispensing and drying the single-s...

Embodiment 3

[0106] (1) Synthesis of probes

[0107] After preparing the single-stranded DNA probe exactly as in Example 1, it was used in the following experiments.

[0108] (2) Probe solvent

[0109] In exactly the same manner as in Example 1, water was used as a probe-soluble solvent.

[0110] (3) Probe soluble substances

[0111] Hexadecylpyridinium chloride (hexadecylpyridinium chloride) was used as a substance that dissolves the probe in an organic solvent. In order to obtain an aqueous solution in which cetylpyridinium chloride was completely dissolved, it was dissolved while stirring well.

[0112] (4) Probe insoluble solvent

[0113] As the probe-insoluble organic solvent, a silane coupling agent (trade name: Y-5187, Japan UNICAR ( Co., Ltd.), isopropyl alcohol, dipropylene glycol.

[0114] (5) Modulation of probe medium

[0115] A sample obtained by dispensing and drying the single-stranded DNA probe of sequence number: 1 in Example 1(1) into 18 nmol was prepared, and 20 μ...

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Abstract

When a probe medium is spotted on a substrate, a probe can be effectively and stably immobilized on the substrate. The probe medium includes a probe capable of specifically binding to a target substance, a medium containing an organic solvent, and a substance for solubilizing the probe in the organic solvent.

Description

technical field [0001] The present invention relates to a probe medium containing a probe capable of specifically binding a target substance and a method for producing the probe medium. The present invention also relates to a probe medium useful for efficiently immobilizing a probe on a substrate, a method for producing the probe medium, and a method for immobilizing a probe using the probe medium. Furthermore, the present invention also relates to a probe-immobilized substrate obtained by immobilizing a probe on a substrate, and a detection element and a detection method for detecting a target substance using the probe-immobilized substrate. Background technique [0002] With the advancement of biotechnology fields such as genetic engineering and molecular biology, it is also possible to diagnose infectious diseases, cancer or genetic diseases at the DNA and RNA levels. As one of tools used in diagnosis using nucleic acids such as DNA and RNA, DNA chips (chips) and DNA arr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/10
CPCC12Q1/68Y10T436/143333C12N15/10
Inventor 冈田良克
Owner CANON KK
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