Preparation method of enzyme enriched active yeast feed
The technology of active yeast and feed is applied in the field of preparation of enzyme-rich active yeast feed, which can solve the problems of small number of live yeast, low enzyme activity of feed yeast, low metabolic activity of yeast cells, etc., and achieve the effect of improving animal production performance.
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Embodiment 1
[0034] 1. Preparation of koji and seed solution
[0035] Rhizopus oryzae (Rhizopus oryzae AS 3.866): purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences. Expanded cultivation process: bran test tube (original species) → triangular flask seeds → bent box seed koji (bran) → Rhizopus oryzae seed koji
[0036] Add 70% water to the bran, cook for 30 minutes under the condition of 0.1Mpa, cool to 35°C and inoculate Rhizopus oryzae cultured in a triangular flask, the inoculum amount is 0.3%, keep warm at 30°C to cultivate the bacteria, after 12 hours the strains germinate and heat up, and culture in the upper curved box , the total cultivation time is 30 hours, and it can be dried out of the room. The drying temperature should not exceed 38°C in the early stage and not exceed 45°C in the later stage. The moisture content of 10% is the Rhizopus oryzae koji species.
[0037] Geotrichum candida seed koji culture
[0038]Geotrichum...
Embodiment 2
[0054] 1. Preparation of koji and seed solution
[0055] Rhizopus oryzae koji culture
[0056] Rhizopus oryzae AS 3.866: purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences. Expanded cultivation process: bran test tube (original species) → triangular flask seeds → bent box seed koji (bran) → Rhizopus oryzae seed koji
[0057] Add 75% water to the bran, cook at 0.15Mpa for 30 minutes, cool down to 35°C and inoculate Rhizopus oryzae cultured in a triangular flask with an inoculum size of 0.3%, keep warm at 30°C to cultivate the bacteria, after 12 hours the strains germinate and heat up, and culture in the upper curved box , the total cultivation time is 36 hours, and it can be dried out of the room. The drying temperature should not exceed 38°C in the early stage and not exceed 45°C in the later stage. The moisture content of 10% is the Rhizopus oryzae koji species.
[0058] Geotrichum candida seed koji culture
[0059] G...
Embodiment 3
[0075] 1. Preparation of koji and seed solution
[0076] Rhizopus oryzae koji culture
[0077] Rhizopus oryzae AS 3.866: purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences. Expanded cultivation process: bran test tube (original species) → triangular flask seeds → bent box seed koji (bran) → Rhizopus oryzae seed koji
[0078] Add 72.5% water to the bran, cook for 30 minutes under the condition of 0.12Mpa, cool to 35°C and inoculate Rhizopus oryzae cultured in a triangular flask, the inoculum amount is 0.3%, keep warm at 30°C to cultivate the bacteria, after 12 hours the bacteria germinate and heat up, and then culture in the upper bent box , the total cultivation time is 33 hours, and it can be dried out of the room. The drying temperature does not exceed 38°C in the early stage and 45°C in the later stage. The moisture content is 10%, which is the Rhizopus oryzae koji species.
[0079] Geotrichum candida seed koji cultu...
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