Endogenic bacterial strain Selection method for preventing potato late blight

A technology for potato late blight and endophytic bacteria, applied in biochemical equipment and methods, microbe measurement/inspection, etc., can solve the problems of unstable control effect and poor colonization ability, and achieve good antibacterial and disease prevention effects , stable and long-lasting effect, good stability

Inactive Publication Date: 2006-12-13
重庆工学院
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AI Technical Summary

Problems solved by technology

It is greatly affected by the environment, so its colonization ability is poor, and it often has a certain control effect in the laboratory, but the control effect is often unstable in field production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] 1. Wash the healthy potato roots, stems and leaves to be separated, dry, soak in 75% alcohol for 2-5 minutes, soak in 0.1% mercuric chloride for 1-2 minutes, wash with sterilized water for 4 times in turn, add sterilized water Grind a small amount, let it stand for 10-15 minutes, and apply the bacterial suspension to the plate with a curved glass rod, and apply two plates in a row. At the same time, coat the plate with sterilized water for washing the separation material for the last time. If colonies grow on this plate later, discard the isolated strain. Culture in a 28°C incubator, pick a single colony for 48 hours and purify to obtain a pure strain, transfer it to a tube and save it for use.

[0025] 2. Plate antagonism test: Inoculate the endophytic bacteria of potato and Phytophthora potato on the rye peptone medium plate relatively, and measure the antibacterial bandwidth after two days. The strains with bacteriostatic effect were screened again on the rye pepton...

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Abstract

A select method for strain preventing and curing endotrophic mycorrhiza in potato. Detect width of bacteriostasis braid and select strains with good effects of endotrophic mycorrhiza, do pot incubation test and field practice, detect effects of prevention and cure and fix planting ability. Detect action of the strain to pathogenetic fungi, and induct antiviral ability to potato. Advanced strain is obtained after the steps.

Description

technical field [0001] The invention relates to a long-term symbiosis between endophytic bacteria and plants, which can slow down the defense reaction of host plants, and is a method for selecting endophytic bacterial strains for potato biological control. Background technique [0002] Most of the biocontrol bacteria for plant diseases are isolated from the soil, rhizosphere or plant surface, while the bacteria with antagonistic effects are determined indoors, and most of them cannot colonize the host surface or body normally when applied outdoors. Factors such as changes in conditions and changes in plant rhizosphere microbial populations under different ecological conditions make their biocontrol effects unstable and sometimes even ineffective. [0003] Production is mainly based on chemical control, which is not only difficult to sustain effective long-term, but also seriously pollutes the environment. Potato is an important crop. The application of pesticides will not o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18
Inventor 王万能全学军陆天健
Owner 重庆工学院
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