Model of mouth cavity biomembrane and method for forming mouth cavity biomembrane

Abstract

An oral biomembrane model equipment is composed of alkali solution container, culture medium container, fermentor, constant-current culture chambers and sample bottle. A process for forming the oral biomembrane includes aseptic connection of said equipment, inoculating experimental bacterial strain into fermentator, continuous culture, regulating pH=7.0, adding artificial saliva as culture medium, culturing, continuing to culture in parallel constant-current culture chambers, and mediating it on hydroxyphosphorite sheet to obtain oral biomembrane.

Description

1. Technical Field The invention relates to an oral biofilm model device and a method for forming an oral biofilm, belonging to the field of biofilm oral medical model instruments or oral medicine research. 2. Background technology As a single-celled life form, bacteria have always used the single-cell state free in liquid medium as the research object in long-term research. This simplifies the influence of the external environment to the greatest extent, and has also obtained a lot of research results. , Laid the foundation for modern microbiology. However, in the natural state, bacteria rarely exist in this form of floating in pure culture. Biofilm is the most common form of survival of bacteria. It refers to a microbial ecological environment formed by a large number of microorganisms gathered in a matrix composed of extracellular polysaccharides on the surface of a solid organic or inorganic medium and cross-linked with each other. In the medical field, biofilm diseases acco...

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Problems solved by technology

At present, the relevant biofilm model reported by Liu Zheng et al. in China (Chinese Journal of Stomatology. 1996; 31(2): 110-112.) is only a simple chemostat bacterial culture system, although it can meet the basic requirements for continuous microbial culture. , but its structure is complicated, and the core component is the continuous static culture of fermenter, which still cannot effectively simulate the continuous flow environment of saliva and other nutrient fluids in the oral cavity, and the control of pollution is not good; while similar foreign products, such as Sissons, are equal to Journal of Dental Research, 1991, 70 (11): 1409-16 reports the constant-thickness biofilm model (Microbiology, 1996, 142, 631-638) that multi-workstation artificial oral cavity system (MAM) and Kinniment et al. The control precision is high, but its structure is complex, and the precision requirements for peripheral equipment are correspondingly high, so it is expensive and the application cost remains high. Although it can simulate the oral environment well, it still cannot be popularized and applied

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