Simplifying cultivating millet variety breeding and its cultivating method
A technology for varieties and millet, applied in the field of simplified cultivation of millet varieties and their supporting cultivation fields, can solve problems such as non-resistance to herbicides, and achieve the effects of improving labor efficiency, improving technical content, and promoting industrialization development.
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Embodiment 1
[0033] Breeding and cultivation of non-herbicide-resistant conventional varieties + cytoplasmic atrazine-resistant near-isogenic lines. The breeding method is to use conventional millet varieties as the male parent and the cytoplasmic atrazine-resistant millet variety as the female parent for sexual crossing. f 1 True hybrids can be identified according to the marker traits of the male parent, such as plant height, panicle shape, anther color, leaf sheath color, etc., or by molecular marker technology. And use conventional millet varieties as reincarnation parent (male parent) and hybrid F 1 Carry out 6 generations of backcrossing with the backcross offspring, spray atrazine before emergence after sowing in the separation generation, and select excellent individual plants with comprehensive traits close to the recurrent parent (male parent) before maturity, until the breeding of the recurrent parent's comprehensive traits is basically the same. near isogenic line. The cytop...
Embodiment 2
[0035] Breeding and cultivation of cytoplasmic atrazine-resistant strain + herbicide-resistant isotype sister line. The breeding method is to use conventional millet varieties as the male parent and the cytoplasmic atrazine-resistant millet variety as the female parent for sexual crossing. f 1 True hybrids can be identified according to the marker traits of the male parent, such as plant height, panicle shape, anther color, leaf sheath color, etc., or by molecular marker technology. f 2 Select excellent individual plants before the generation matures, and identify atrazine resistance at the seedling stage for part of the seeds harvested from each individual plant, eliminate individual plants that are not resistant to herbicides at all or all are resistant to herbicides, and leave isolated individual plants with resistance . from F 3 At the beginning of the generation, individual plants were selected in excellent families before maturity, and part of the seeds harvested fro...
Embodiment 3
[0037] Breeding and cultivation of conventional millet variety + near isogenic line resistant to naprozine. The breeding method is to cross the conventional millet variety with the anti-napkin millet variety (reciprocal crossing is acceptable). f 1 Generation 3-5 leaf stage (3-leaf stage is the best) spray Napozine (15% EC, 0.15ml / m 2 , mixed with water 75ml), kill the false hybrids that are not resistant to Napo, and the surviving ones are true hybrids. Using conventional millet varieties as recurrent parent (male parent), and hybrid F 1 Backcross 6 generations with backcross offspring. The 3-5 leaf stage (the 3rd leaf stage is the best) of the separation generation was sprayed with Napozine, and the excellent individual plants with comprehensive traits close to the recurrent parents were selected before maturity, until the near-isogenic lines with the comprehensive traits basically consistent with the recurrent parents were bred. The near-isogenic line of anti-naprozine ...
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