Method for quickly breeding high quality sprout of butterfly orchid

A technique for Phalaenopsis and seedlings, applied in the field of rapid propagation of high-quality Phalaenopsis seedlings, to achieve the effects of long flowering period, broad market prospects, and simple and affordable technology

Inactive Publication Date: 2006-06-07
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, many studies have been carried out on the aseptic sowing and tissue culture of Phalaenopsis at home and abroad, but there is no report on the rapid propagation and large-scale production of Phalaenopsis high-quality seedlings through the cluster bud approach.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Material: Phalaenopsis Taida Salu (Phalaenopsis Taida Salu "Sunset Red")

[0016] 2. Disinfection of explants: Take the pedicel of the sunset red flower bud, rinse it with tap water, cut the segment with dormant bud 2-3 cm, soak it with 70% alcohol on the ultra-clean workbench for 1 minute, and then Sterilize with 0.1% (percentage by weight) mercuric chloride solution for 15 minutes, rinse with sterile water for 5 times, blot dry with sterile absorbent paper, cut off 0.5 cm at both ends of the incision, and cut 1-2 cm long with dormant buds Cut sections were inoculated on pedicel seedling induction medium.

[0017] 3. Induction of pedicel seedlings: the induction medium of pedicel seedlings is VW+6-benzylpurine 10 mg / liter+naphthalene acetic acid 0.5 mg / liter+sodium citrate 150 mg / liter, 15-day dormant buds start to grow, and in culture When the temperature is (28±2)℃, pedicel seedlings with leaves are mostly formed.

[0018] 4. Proliferation of clustered buds: whe...

Embodiment 2

[0022] The Phalaenopsis variety Super Red (Dtps Super-Red) with high ornamental value obtained from self-cultivation is an explant, and the basic operation method is consistent with Example 1. However, the medium composition is slightly different. The induction medium of its pedicel seedlings is VW+6-benzylpurine 15 mg / liter+naphthalene acetic acid 1.0 mg / liter+sodium citrate 100 mg / liter, and the cluster bud proliferation medium is MS+6-benzylpurine 15 mg / liter + naphthalene acetic acid 0 mg / liter + sodium citrate 100 mg / liter + 15% coconut water + 30 g / liter of sucrose, the survival rate of transplanted test-tube plantlets can reach 100%.

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Abstract

The invention relates to a method for rapid propagation of high-quality seedlings of Phalaenopsis and a used culture medium. Phalaenopsis, also known as Phalaenopsis, is one of the most widely cultivated and popular species in Orchidaceae, and is known as the "Queen of Orchids". It is a very famous and precious indoor flower. There are few Phalaenopsis varieties with self-produced intellectual property rights in mainland my country, and the Phalaenopsis seedlings produced are of poor quality. The current main propagation method of Phalaenopsis, the offspring will be separated, which cannot maintain the excellent traits of the mother and the identity of the seedlings, or will increase the coefficient of variation of the seedlings. The present invention uses the pedicel segment of Phalaenopsis as explant, utilizes unique culture medium to induce pedicel fungus, and then uses the stem tip or stem section of the pedicel seedling to proliferate by clustering buds to produce high-quality Phalaenopsis seedlings. The method and technology are simple and affordable, practical and of high application value, and provide an effective way to meet the needs of the market.

Description

technical field [0001] The invention relates to a method for rapid propagation of high-quality seedlings of Phalaenopsis and a used culture medium. Background technique [0002] Phalaenopsis, also known as Phalaenopsis, is one of the most widely cultivated and popular species in Orchidaceae, and is known as the "Queen of Orchids". There are more than 40 native species of this genus in the world, and there are 6 native species in my country. However, due to the excessive harvesting of wild resources, most of the wild native species are difficult to find now, and the original species of Phalaenopsis have also been removed. The World Wildlife Conservation Organization lists it as one of the world's top ten endangered plant species. At present, most of the Phalaenopsis ornamental species with various poses and with different expressions on the market and large and colorful flowers are hybrids, and can be hybridized with other genera to form intergenus hybrids, such as intergenus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/00A01G7/00
Inventor 曾宋君陈之林段俊陈建通
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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