Method for quickly breeding high quality sprout of butterfly orchid
A technique for Phalaenopsis and seedlings, applied in the field of rapid propagation of high-quality Phalaenopsis seedlings, to achieve the effects of long flowering period, broad market prospects, and simple and affordable technology
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Embodiment 1
[0015] 1. Material: Phalaenopsis Taida Salu (Phalaenopsis Taida Salu "Sunset Red")
[0016] 2. Disinfection of explants: Take the pedicel of the sunset red flower bud, rinse it with tap water, cut the segment with dormant bud 2-3 cm, soak it with 70% alcohol on the ultra-clean workbench for 1 minute, and then Sterilize with 0.1% (percentage by weight) mercuric chloride solution for 15 minutes, rinse with sterile water for 5 times, blot dry with sterile absorbent paper, cut off 0.5 cm at both ends of the incision, and cut 1-2 cm long with dormant buds Cut sections were inoculated on pedicel seedling induction medium.
[0017] 3. Induction of pedicel seedlings: the induction medium of pedicel seedlings is VW+6-benzylpurine 10 mg / liter+naphthalene acetic acid 0.5 mg / liter+sodium citrate 150 mg / liter, 15-day dormant buds start to grow, and in culture When the temperature is (28±2)℃, pedicel seedlings with leaves are mostly formed.
[0018] 4. Proliferation of clustered buds: whe...
Embodiment 2
[0022] The Phalaenopsis variety Super Red (Dtps Super-Red) with high ornamental value obtained from self-cultivation is an explant, and the basic operation method is consistent with Example 1. However, the medium composition is slightly different. The induction medium of its pedicel seedlings is VW+6-benzylpurine 15 mg / liter+naphthalene acetic acid 1.0 mg / liter+sodium citrate 100 mg / liter, and the cluster bud proliferation medium is MS+6-benzylpurine 15 mg / liter + naphthalene acetic acid 0 mg / liter + sodium citrate 100 mg / liter + 15% coconut water + 30 g / liter of sucrose, the survival rate of transplanted test-tube plantlets can reach 100%.
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