Method for extracting tetrodotoxin from globefish livers
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of tetrodotoxin and fish liver, applied in the direction of organic chemistry, can solve the problem of low recovery rate, and achieve the effect of improving recovery rate, easy access, and reducing the loss of biological activity
Inactive Publication Date: 2006-03-15
OCEAN UNIV OF CHINA
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Using this method, 10g tetrodotoxin can be obtained from 1000kg ovary, and the recovery rate is very low
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0009] 1. Take out 25 kg of puffer fish liver from the freezer, chop it with a slicer, put it into a polyethylene bucket with a volume of 80 liters, add 50 liters of deionized water, soak at low temperature (0°C-15°C) for 20 hours, Stir at the right time during the soaking process, and the speed of stirring is relatively slow. The next day, the extraction liquid is released from the lower mouth of the barrel, and the discharge liquid is filtered through 350 mesh gauze. This is the first toxin extraction solution, which is pumped into the freezer through a peristaltic pump and frozen for 4 hours for later use. Repeat soaking 3 times again to obtain 185 liters of crude venom. Stop immersion after the fifth extraction liquid is tested to be non-toxic by the mouse method.
[0010] 2. Put the crude venom above in the freezer and then form a flocculent precipitate. Use a peristaltic pump to suck out the upper clarified liquid and put it into a heating pot. Filter the bottom sedimen...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The method for extracting tetrodotoxin from liver of globefish includes the following steps: cutting liver of globefish, soaking at low temp., stirring, filtering, freezing, repeatedly soaking for several times to obtain crude venom, freezing and standing still, precipitating, heating and boiling, discharging out venom, freezing, and separating out supernatant venom, regulating pH value, adding active carbon, stirring, filtering, mixing cation 110 resin and said clear liquor, stirring, filtering, making said clear liquor pass through cation 110 resin, saturating and using acid to make elution, collecting eluant, regulating pH value, cooling, standing still, crystallizing, sucking out mother liquor, filtering, more regulating pH, freezing, standing still, crystallizing and repeatedly regulating pH for several times to obtain white powder crystal.
Description
technical field [0001] The invention relates to a method for extracting high-purity tetrodotoxin from puffer fish liver. Background technique [0002] Tetrodotoxin (TTX) is also known as Spheridine, Fugupoison, etc. TTX is an alkaloid toxin that was first isolated from puffer fish. The pharmacy masterpiece "Compendium of Materia Medica" of the Ming Dynasty in my country introduced the toxicity of puffer fish in more detail. TTX is one of the most poisonous non-protein substances known to mankind so far. Patients poisoned by tetrodotoxin will immediately have serious symptoms and endanger the human body. Life, the mortality rate is higher (generally 60%). But TTX has important practical application value. In addition to being used as a drug, it also has attractive application prospects in drug addiction, antiarrhythmia, respiratory depression, prevention of renal failure, cardiovascular, analgesia, local anesthesia, etc. TTX is a white crystalline solid, needle-like to diam...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.