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Abnormal decelled bone based material and its preparation

A decellularized bone matrix and heterogeneous technology, applied in the field of heterogeneous bone matrix materials and bone tissue engineering materials, can solve the problems of high cost, unstable performance, poor osteogenesis ability, etc., and achieve good mechanical strength and stiffness, good Osteoconductivity, the effect of eliminating worries

Inactive Publication Date: 2005-12-07
INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inorganic bone does not contain organic components, so it is loose and brittle, and does not have basic mechanical strength; the latter lacks bone mineral, so its texture is soft and does not have basic mechanical stiffness
These deficiencies all limit their application in bone tissue engineering [Liu Jianzhong, Wang Zhen, Hu Yunyu, etc. "Complications after Allogeneic Bone Joint Transplantation to Repair Limb Segmental Bone Defects", Chinese Journal of Surgery, 2000, 38(5): 332 ~335]
However, artificial synthetic materials have problems such as high cost, unstable performance, low mechanical strength, weak osteoinductive activity, and poor osteogenic ability.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: A preparation method of a heterogeneous decellularized bone matrix material: take fresh ribs of pigs weighing 70-100Kg, remove soft tissue and bone marrow, and make blocks according to needs (for the verification experiment described above, this Example size is 1cm 3 about). Rinse with tap water for no less than 30 minutes; then soak in distilled water for 45 minutes. After taking it out to dry, at room temperature, in the concentration of 30% H 2 o 2 React for 12 hours to destroy cells and interstitial proteins. Soak in distilled water for 40 minutes. After it was taken out to dry, it was degreased in methanol-chloroform solution with a concentration of 1:1 at room temperature for 24 hours. Then dissolve with absolute ethanol to remove excess chloroform for 24 hours. After taking it out to dry, react in a solution containing 0.8N NaOH, 2% Triton-X100, and 5% Dispase enzyme under the condition of 37°C±1.5°C for 20 hours to remove the cells. Then, disso...

Embodiment 2

[0022] Example 2: A preparation method of a heterogeneous decellularized bone matrix material: take fresh ribs of pigs weighing 70-100Kg, remove soft tissue and bone marrow, and make blocks according to needs (for the verification experiment described above, this Example size is 1cm 3 about). Rinse with tap water for no less than 30 minutes; then soak in distilled water for 50 minutes. After taking it out to dry, at room temperature, in the concentration of 30% H 2 o 2 React for 12 hours to destroy cells and interstitial proteins. Soak in distilled water for 35 minutes. After it was taken out to dry, it was degreased in methanol-chloroform solution with a concentration of 1:2 for 18 hours at room temperature. Then dissolve with absolute ethanol to remove excess chloroform for 32 hours. After taking it out to dry, react in a solution containing 0.2N NaOH, 1.5% Triton-X100, and 2.5% pepsin at 37°C±1.5°C for 36 hours to remove the cells. Then, dissolve and thoroughly remov...

Embodiment 3

[0023] Example 3: A preparation method of a heterogeneous decellularized bone matrix material: take fresh ribs of pigs weighing 70-100Kg, remove soft tissue and bone marrow, and make blocks according to needs (for the verification experiment introduced above, this Example size is 1cm 3 about). Rinse with tap water for no less than 30 minutes; then soak in distilled water for 60 minutes. After taking it out to dry, at room temperature, in the concentration of 30% H 2 o 2 React for 12 hours to destroy cells and interstitial proteins. Soak in distilled water for 30 minutes. After it was taken out to dry, it was degreased in a methanol-chloroform solution with a concentration of 1:4 for 12 hours at room temperature. Then dissolve with absolute ethanol to remove excess chloroform for 48 hours. After taking it out to dry, react in a solution containing 1.0N NaOH, 4.5% Triton-X100, and 8% trypsin at 37°C±1.5°C for 18 hours to remove the cells. Then, dissolve and thoroughly rem...

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Abstract

A cell-removed heterogeneous bone matrix is prepared from the rib and extremity bone of pig through physical and chemical processing, and removing cells and the heterogeneous protein from tissue by use of proteolytic enzyme and Triton-X100. Its advantages are natural components and biologic chemical strength, low antigenicity, high compatibility and bone conductivity, and low cost.

Description

technical field [0001] The present invention relates to substitutes required for bone defect and nonunion repair and materials for constructing bone tissue engineering, [0002] Specifically, xenogeneic bone matrix materials. Background technique [0003] The matrix materials used for bone defect repair and bone tissue engineering can be divided into the following categories: natural bone and its derivatives; artificial synthetic materials; composites composed of various materials, etc. Among them, natural bone and its derivatives (bone processed by various methods) have a network pore system close to autologous bone, the composition and structure meet physiological requirements, and have good cell compatibility, which is conducive to the growth of seed cells. It has been widely used in bone tissue engineering. According to its source, it is divided into allograft bone and xenograft bone. In contrast, xenogeneic bone is relatively abundant and cheap. However, both have i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
Inventor 孙新君王正国朱佩方
Owner INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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