Protein nanostructure as well as self-assembly method and application thereof

A nanostructure and self-assembly technology, applied in the field of nanomaterials, can solve the problems of high reaction temperature, insoluble in water, and inapplicability of bio-based materials, etc., and achieve the effect of low cost

Pending Publication Date: 2022-08-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the nanofibers prepared by electrospinning are usually stacked together in a film shape, and most of them are insoluble in water; the reaction temperature of the chemical vapor deposition method is high, which is not suitable for bio-based materials; the reaction temperature of the single self-assembly method Moderate, suitable for bio-based materials, but the formed nanofibers are usually solid

Method used

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  • Protein nanostructure as well as self-assembly method and application thereof
  • Protein nanostructure as well as self-assembly method and application thereof
  • Protein nanostructure as well as self-assembly method and application thereof

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preparation example Construction

[0042] An aspect of the embodiments of the present invention provides a method for preparing a self-assembled core-shell structural protein nanoparticle and a hollow structural protein nanofiber, including:

[0043] Prepare bulk nanobubbles, and uniformly mix the solution containing bulk nanobubbles with protein;

[0044] Adjust the pH value, protein concentration and bulk nanobubble concentration of the reaction system, change the hydrophobic interaction and electrostatic interaction strength between the protein and bulk nanobubble, and make the protein stably adsorbed in the gas of bulk nanobubble. - At the liquid interface, self-assembled core-shell structural protein nanoparticles are formed;

[0045] Heating the obtained mixture for a short time to partially denature the protein to form stable self-assembled nucleocapsid protein nanoparticles;

[0046] Elevating the reaction temperature and prolonging the heating time completely denatures the protein and forms self-assem...

Embodiment 1

[0092] (1) Put ultrapure water with a conductivity of 18.2 MΩ·cm in a closed container, and pass pure air into the ultrapure water at a flow rate of 350 mL / min for 10 min to make the pure air content in the water. reach saturation.

[0093] (2) Reduce the pressure of the airtight container to 5.0 kPa, and then gradually pressurize to restore the pressure inside the container to atmospheric pressure. After repeated decompression and pressure mixing, bulk nanobubbles are formed in the solution.

[0094] (3) Mix pea protein isolate and ultrapure water thoroughly, and continue stirring at 500 rpm for 2 h at 25 °C to prepare a solution with a protein concentration of 1.2 mg / mL, and then stand at 4 °C for 24 h to make pea protein isolate. Fully hydrated, then centrifuged at 4 °C and 8000 rpm for 20 min to collect the soluble pea protein isolate solution.

[0095] (4) Add the bulk nanobubble aqueous solution in step (2) dropwise to the pea protein isolate solution obtained in step (...

Embodiment 2

[0101] (1) Put ultrapure water with a conductivity of 18.2 MΩ·cm in a closed container, and pass pure air into the ultrapure water at a flow rate of 200 mL / min and keep it for 20 min to make the pure air content in the water. reach saturation.

[0102] (2) Reduce the pressure of the airtight container to 4.0 kPa, and then gradually pressurize to restore the pressure inside the container to atmospheric pressure. After repeated decompression and pressure mixing, bulk nanobubbles are formed in the solution.

[0103] (3) Mix pea protein isolate and ultrapure water thoroughly, and continue stirring at 500 rpm for 2 h at 20 °C to prepare a solution with a protein concentration of 3.0 mg / mL, and then stand at 8 °C for 36 h to make pea protein isolate. Fully hydrated, then centrifuged at 4 °C and 4000 rpm for 40 min to collect the soluble pea protein isolate solution.

[0104] (4) Add the bulk nanobubble aqueous solution in step (2) dropwise to the pea protein isolate solution obtain...

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Abstract

The invention discloses a protein nanostructure and a self-assembly method and application thereof. The invention particularly discloses a preparation method for forming core-shell nanoparticles and hollow nanofibers by regulating protein self-assembly through bulk-phase nanobubbles. According to the method, the bulk-phase nanobubble is spontaneously adsorbed on the surface of the bulk-phase nanobubble by utilizing the hydrophobic property of the bulk-phase nanobubble as gas and the hydrophilic property of charge carried on the surface of the nanobubble and combining the inherent amphipathy of protein and the characteristic that the protein is easy to adsorb on a gas-liquid interface; the core-shell structure nanoparticles with different nanometer sizes are formed. The method for regulating and controlling the self-assembled protein nanostructure is simple in preparation process, easy to operate, free of complex equipment, low in cost, safe, reliable and easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a bulk nanobubble regulating protein self-assembly nanoparticle and nanofiber, and a self-assembly preparation method and application thereof. Background technique [0002] Nanoparticles and nanofibers are important delivery systems for encapsulating biologically active functional factors or drugs, and have received extensive attention from researchers in recent years. The nano-size effect gives particles and fibers better stability, increases their residence time in the gastrointestinal tract digestion process, and increases their tissue penetration and uptake by epithelial cells. However, the digestion and absorption behaviors of different nanostructures are significantly different, so the selection of appropriate nanostructures is of great significance in improving the release and absorption processes of functional factors or drugs. Compared with chemically ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/00C08L89/00D01F4/00B82Y40/00
CPCC08J3/00D01F4/00B82Y40/00C08J2389/00
Inventor 刘东红闫天一王文骏徐恩波丁甜程焕叶兴乾
Owner ZHEJIANG UNIV
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