Application of lung cough in preparation of products for preventing and treating breast cancer

A technology for breast cancer and breast cancer cells, applied in the field of biopharmaceuticals, can solve problems such as undocumented technical content related to breast cancer treatment or prevention, and achieve the effects of increasing market share, inhibiting vitality, and improving application prospects.

Pending Publication Date: 2022-08-09
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prior art does not record the technical content related to the treatment or prevention of lung cancer and breast cancer

Method used

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  • Application of lung cough in preparation of products for preventing and treating breast cancer
  • Application of lung cough in preparation of products for preventing and treating breast cancer
  • Application of lung cough in preparation of products for preventing and treating breast cancer

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preparation example Construction

[0030] The present invention also provides a preparation method of the above-mentioned medicine, comprising the following steps: directly preparing the lung cancer into a pharmaceutical preparation for preventing and treating breast cancer, or compounding the lung cancer and other medicines for preventing and treating breast cancer to prepare into a compound pharmaceutical preparation, or the active ingredients of the pulmonary cough and a pharmaceutically acceptable carrier are prepared into a pharmaceutical preparation for preventing and treating breast cancer.

[0031] In the preparation method of the pharmaceutical preparation of the present invention, the contents that are not described in detail, such as auxiliary materials, how to prepare, etc., are all conventional choices in the art.

Embodiment 1

[0034] MCF-7 cells, MDA-MB-231 cells, MDA-MB-468 cells, and 4T1 cells were collected in log phase, according to 6 × 10 3 The density of cells / well was inoculated in 96-well plates, and cultured overnight. After the cells adhered to the wall, sample solutions of different concentrations (lung Physiological saline solution of the active ingredient of Like Capsule (drug after removing the capsule shell), Feilike Capsule was produced by Guizhou Jianxing Pharmaceutical Co., Ltd.), and normal saline was used as the control group. After 72 hours of drug action, 10 μl of MTT with a concentration of 5 mg / ml was added to each well, incubated at 37°C for 4 hours, the supernatant was discarded, and 160 μl of DMSO was added to each well to dissolve. After the blue-purple crystal formazan was completely dissolved, the absorbance value OD ( 490nm).

[0035] Calculation method of inhibition rate: inhibition rate=(OD value of control group-OD value of treatment group) / OD value of control grou...

Embodiment 2

[0038] MDA-MB-231 cells were pressed 4 × 10 5 The amount of cells / well was inoculated in a 60mm circular petri dish for culture. After the cells adhered to the wall, sample solutions of different concentrations (0.25mg / ml, 0.5mg / ml, 1mg / ml, 2mg / ml) were added (lung Physiological saline solution of the active ingredient of Like Capsule (drug after removing the capsule shell), Feilike Capsule was manufactured by Guizhou Jianxing Pharmaceutical Co., Ltd., was used for 24h, and normal saline was used as the control group. The morphological changes of cells were observed under an inverted microscope.

[0039] The result is as figure 1 As shown, the number of cells decreased significantly with increasing drug concentration. The cell morphology changed, especially at high concentration, the cell shape changed from long spindle to round, and the cells were swollen.

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Abstract

The invention provides application of lung cough in preparation of a product for preventing and treating breast cancer, and belongs to the technical field of biological pharmacy. It is found for the first time that lung force cough can effectively treat breast cancer, and the lung force cough has the effects of inhibiting the activity of breast cancer cells, inhibiting proliferation of the breast cancer cells, promoting apoptosis of the breast cancer cells and changing the morphology of the breast cancer cells, so that the effect of treating the breast cancer is achieved. The lung cough inhibits proliferation of breast cancer cells, has concentration dependence, has obvious influence on the human breast cancer cell cycle, and can retard the breast cancer cell cycle in the S phase, and the higher the drug concentration is, the more obvious the retardation is.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and particularly relates to the application of Feilike in the preparation of products for preventing and treating breast cancer. Background technique [0002] Pulmonary cough is a kind of Chinese patent medicine. The main ingredients include Scutellaria baicalensis, Qianhu, Baibu, safflower gentian, sycamore root, Hedyotis diffusa and red tube medicine. Currently, there are two medicinal formulations of Feilike in circulation, Feilike Mixture and Feilike Capsule. Feilike Capsules contain yellowish-brown to brown powders and granules with a bitter taste. The clinical efficacy of Lung Cough is relieving cough and relieving asthma, clearing heat and detoxifying, smoothing Qi and removing phlegm. The prior art does not record the technical content related to the treatment or prevention of lung cancer and breast cancer. SUMMARY OF THE INVENTION [0003] In view of this, the purpose of t...

Claims

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Application Information

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IPC IPC(8): A61K36/904A61P35/00
CPCA61K36/539A61K36/23A61K36/904A61K36/515A61K36/185A61K36/748A61K36/28A61P35/00A61K2300/00Y02A50/30
Inventor 杨珏李艳梅张知音邱剑飞赵鹏韦学耐
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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