Construction method and application of sheep chronic cold stress model

A construction method and technology of cold stress, which is applied in the field of sheep chronic cold stress model construction, can solve the problems of large temperature difference, inaccurate temperature control, and unobjective response to cold stress behavior of sheep, etc., to achieve high quality Effect

Pending Publication Date: 2022-08-05
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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  • Claims
  • Application Information

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Problems solved by technology

However, the temperature control is not precise enough, the daytime temperature fluctuates continuously during the test period, and the temperature difference between the maximum temperature and the minimum temperature in the daytime is very large. At the same time, the influence of seasonal changes in the nutritional value of forage on the cold stress model is ignored. Therefore, it is not objective enough to respond to the cold stress behavior of sheep

Method used

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  • Construction method and application of sheep chronic cold stress model
  • Construction method and application of sheep chronic cold stress model
  • Construction method and application of sheep chronic cold stress model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The present embodiment provides a method for constructing a sheep chronic cold stress model, which includes the following steps:

[0036] (1) Selection of research subjects and experimental design;

[0037] Twenty-four Hu sheep aged 4-5 months, with an average initial body weight of 35.0±0.52kg and in good health were selected; they were divided into 2 groups on average, including the control group (constant temperature of 20°C, maintained for 41 days) and the chronic cold stress group ( Including 17 days of continuous gradient cooling and 24 days as the formal test period), the test period is 41 in total.

[0038] (2) Establishment of sheep chronic cold stress model.

[0039] Sheep constant temperature cold room adopts continuous gradient cooling method (the average decrease of 5°C every 2 days), the specific temperature changes are 20°C, 15°C, 10°C, 5°C, 0°C, -5°C, -10°C, -15°C , -20 ℃, the temperature is adjusted at 9 am each time, the target temperature is reached...

Embodiment 2

[0043] Compared with Example 1, the only difference is that in step (2), the rest of the steps are the same.

[0044] Step (2) Establishment of a sheep chronic cold stress model.

[0045] The sheep constant temperature cold room adopts a continuous gradient cooling method (an average decrease of 4 °C every 2 days), and the specific temperature changes are 20 °C, 16 °C, 12 °C, 8 °C, 4 °C, 0 °C, -4 °C, -8 °C, -12°C, -16°C, -20°C, the temperature is adjusted at 9 am each time, the target temperature is reached within 30 minutes, the time interval for each temperature adjustment is 2 days, and the final temperature is maintained at -20°C for 24 days.

Embodiment 3

[0047] Compared with Example 1, the only difference is that in step (2), the rest of the steps are the same.

[0048] Step (2) Establishment of a sheep chronic cold stress model.

[0049] Sheep constant temperature cold room adopts continuous gradient cooling method (the average decrease of 6℃ every 2 days), the specific temperature changes are 25℃, 19℃, 13℃, 7℃, 1℃, -4℃, -10℃, -16℃ , -20 ℃, the temperature is adjusted at 9:00 am each time, the target temperature is reached within 30 minutes, the time interval for each temperature adjustment is 2 days, and the final temperature is maintained at -20 ℃ for 24 days.

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Abstract

The invention discloses a construction method and application of a sheep chronic cold stress model, and relates to the technical field of animal model construction. The method comprises the following steps: in a breeding room for breeding to-be-treated sheep, continuously cooling the temperature in the breeding room to-35 DEG C to-20 DEG C in a gradient manner, and keeping the constant temperature for 20-25 days. The construction method of the sheep chronic cold stress model provided by the invention is not influenced by day and night temperature difference and seasonal change of pasture nutritional value, and the temperature can be accurately controlled at any time, so that the sheep chronic cold stress model with higher quality is provided, and an animal model basis is provided for subsequent research on cold stress reaction behaviors of sheep.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a construction method and application of a sheep chronic cold stress model. Background technique [0002] Cold stress is one of the important factors restricting livestock production. In northern China, the winter is cold and long, and the growing season of pasture is short. The low temperature in winter often induces a series of stress responses in livestock (such as indigestion, diarrhea and other diseases), resulting in the decline of livestock production performance, extreme low temperature events (large snow disaster) and even lead to a large number of livestock freezing to death. The researchers found that when the ambient temperature was between -18°C and 0°C, the maintenance needs of a 35kg sheep increased by 3.6MJ / kg for every 1°C decrease in temperature. 0.75 . Equivalent to every 1℃ decrease in temperature, sheep lose 5.0-6.0g of fat per day. Some...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02Y02A40/70
Inventor 姬凯茜杨果伍修锟梁艳萍焦丹冯梦雨贾玉珍丛海涛
Owner NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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