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Joint detection kit for feline herpes and feline calicivirus

A technology for feline calicivirus and feline herpesvirus, which is applied in the field of combined detection kits for feline herpes and feline calicivirus, can solve the problems of time-consuming, time-consuming, inability to meet detection requirements, etc., to ensure specificity and reduce interference the effect of competition

Active Publication Date: 2022-08-02
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Serological detection includes serum neutralization test, immunofluorescence or enzyme-linked immunoassay, etc. These methods are relatively time-consuming; virus detection methods include electron microscopy, RT-PCR, fluorescent quantitative RT-PCR, etc., but these detection methods are time-consuming , and all require expensive instruments and equipment and professional technicians to operate, which cannot meet the detection requirements of non-laboratory and other grass-roots field conditions

Method used

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  • Joint detection kit for feline herpes and feline calicivirus
  • Joint detection kit for feline herpes and feline calicivirus
  • Joint detection kit for feline herpes and feline calicivirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A combined detection kit for feline herpes and feline calicivirus, such as Figure 1 to Figure 3As shown in common, it includes a test card 2, a sample diluent bottle 4 (containing a sample treatment solution) and a sterile cotton swab 5 (packaged in a packaging box 1). The test card 2 is provided with a test strip 3 inside. The paper strip 3 includes a bottom plate 30, a nitrocellulose membrane 31 is arranged on the bottom plate 30, a water absorption pad 32 is arranged above the nitrocellulose membrane 31, and a gold-labeled antibody reaction pad 33 is arranged below the nitrocellulose membrane 31. A sample pad 34 is arranged below the reaction pad 33, and the gold-labeled antibody reaction pad 33 is provided with a feline herpesvirus monoclonal antibody-colloidal gold complex coating and a feline calicivirus monoclonal antibody-colloidal gold complex coating. Calicivirus monoclonal antibody-colloidal gold complex coating 331 is located above feline herpes virus monoc...

Embodiment 2

[0036] The difference from Example 1 is:

[0037] The isolation pad refers to coating the isolation pad treatment solution on the glass fiber and drying it at 37°C for 8 hours; the isolation pad treatment solution contains 0.3wt% Triton X-100 and 0.4wt% Triton X-100 per 1000mL of PBS buffer. Formulated with BSA, the concentration of PBS buffer is 25mM, pH is 7.4.

[0038] The nitrocellulose membrane is provided with a detection line T1 coated with a feline calicivirus monoclonal antibody, a detection line T2 coated with a feline herpes virus monoclonal antibody, and a quality control line coated with a goat anti-cat IgG antibody. Detection line T1, detection line T2 and quality control line refer to diluting feline calicivirus monoclonal antibody to 1.0mg / mL, feline herpes virus monoclonal antibody to 0.85mg / mL, and goat anti-cat IgG with diluent, respectively. To 1.8mg / mL, spray on the nitrocellulose membrane in turn, and then dry.

[0039] The diluent is prepared by contai...

Embodiment 3

[0043] The difference from Example 1 is:

[0044] The isolation pad refers to coating the isolation pad treatment solution on the glass fiber and drying it at 37°C for 8 hours; the isolation pad treatment solution contains 0.4wt% Triton X-100 and 0.5wt% Triton X-100 per 1000mL of PBS buffer. It is formulated with BSA, the concentration of PBS buffer is 25 mM, and the pH is 7.6.

[0045] The nitrocellulose membrane is provided with a detection line T1 coated with a feline calicivirus monoclonal antibody, a detection line T2 coated with a feline herpes virus monoclonal antibody, and a quality control line coated with a goat anti-cat IgG antibody. The detection line T1, the detection line T2 and the quality control line refer to the dilution of feline calicivirus monoclonal antibody to 1.2 mg / mL, feline herpes virus monoclonal antibody to 1.0 mg / mL, goat anti-cat The IgG was diluted to 2.0 mg / mL, sprayed on the nitrocellulose membrane in turn, and dried.

[0046] The diluent is...

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Abstract

The invention relates to the technical field of biological detection, in particular to a feline herpes and feline calicivirus combined detection kit which comprises a detection card, a sample diluent and a sterile cotton swab, a test strip, a test strip nitrocellulose membrane, a water absorption pad, a gold-labeled antibody reaction pad and a sample pad are arranged in the detection card, a feline herpesvirus monoclonal antibody-colloidal gold compound coating and a feline calicivirus monoclonal antibody-colloidal gold compound coating are arranged on the gold-labeled antibody reaction pad, and the feline calicivirus monoclonal antibody-colloidal gold compound coating is positioned above the feline herpesvirus monoclonal antibody-colloidal gold compound coating; and an isolation pad is arranged between the feline calicivirus monoclonal antibody-colloidal gold compound coating and the feline herpes virus monoclonal antibody-colloidal gold compound coating. The kit is simple to operate and short in detection time, the detection efficiency is greatly improved, in addition, expensive instruments and equipment are not needed for detection, and the detection cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a combined detection kit for feline herpes and feline calicivirus. Background technique [0002] Feline upper respiratory tract disease is a common feline disease. Common feline upper respiratory tract diseases include feline herpes virus (FHV-1), feline leukemia virus (FeLV), and feline calicivirus (FCV). Feline herpesvirus (FHV-1), also known as feline rhinotracheitis virus (felinerhinotracheitis virus), belongs to the family alpha-herpesviridae, and is a double-stranded DNA virus with an envelope. The virus mainly affects young cats and is transmitted through direct contact. It is clinically characterized by keratoconjunctivitis, upper respiratory tract infection and miscarriage, but the upper respiratory tract symptoms are the main symptoms. The morbidity rate is as high as 100% and the mortality rate can reach 50%. Feline calicivirus (FCV) is a member of the ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/58G01N33/558G01N33/543G01N33/52
CPCG01N33/577G01N33/56994G01N33/56983G01N33/587G01N33/558G01N33/54346G01N33/52G01N2333/03G01N2333/08G01N2469/10Y02A50/30
Inventor 杨帆陈秀岚杨致亭朱衍杰杨明霞
Owner 山东康华生物医疗科技股份有限公司
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