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Plasma sample pretreatment method for improving freeze-thaw stability of paclitaxel liposome

A freeze-thaw stability and sample pretreatment technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as increasing the single work intensity of operators, improving the stability of paclitaxel liposomes, and affecting the results of analysis and detection. , to improve sampling and detection throughput, improve freeze-thaw stability, and ensure freeze-thaw stability.

Active Publication Date: 2022-08-02
WUXI APPTEC SUZHOU
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Problems solved by technology

However, pretreatment such as solid-phase extraction is carried out immediately after plasma is obtained. The operation time of this series of steps is long and the operation is relatively cumbersome. Continuous work for a long time not only increases the single work intensity of the operator, but also easily leads to operation failure. Mistakes will affect the results of analysis and detection; in addition, avoiding repeated freezing and thawing cannot fundamentally improve the stability of paclitaxel liposomes. Innovative improvements in processing methods

Method used

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  • Plasma sample pretreatment method for improving freeze-thaw stability of paclitaxel liposome
  • Plasma sample pretreatment method for improving freeze-thaw stability of paclitaxel liposome
  • Plasma sample pretreatment method for improving freeze-thaw stability of paclitaxel liposome

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Embodiment 1

[0040]1. Set up the experimental group. The experimental group includes two parallel experiments, namely parallel group 1 and parallel group 2. The specific operations are as follows:

[0041] A. Whole blood sample collection: The blood collection tube is pre-cooled at 4 ℃ in advance. Whole blood is collected from the poisonous experimental dog. The blood collection location includes but not limited to the jugular vein or other suitable sites. In the blood collection tube with K2 EDTA anticoagulant, gently invert the blood collection tube containing the whole blood sample several times to mix the whole blood sample with the anticoagulant and store it in wet ice; blood samples can be kept in wet ice Stabilization for at least 1 hour, in addition to nitrogen sparging to improve liposome stability in blood samples.

[0042] B. Plasma sample collection: Within 1 hour, take the whole blood sample of step 1, centrifuge at 2800 xg, 4 ℃ for 10 minutes to obtain a plasma sample; adjust...

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Abstract

The invention discloses a plasma sample pretreatment method for improving freeze-thaw stability of paclitaxel liposome, which comprises the following steps: collecting a whole blood sample, treating the collected whole blood sample into a plasma sample, treating the plasma sample with a protease inhibitor, freezing the treated plasma sample, carrying out solid-phase extraction and protein precipitation pretreatment on the frozen and thawed plasma sample, and finally determining the freeze-thaw stability of the paclitaxel liposome by adopting an ultra-high performance liquid chromatography-mass spectrometry. And detecting the content of liposome paclitaxel and the content of free paclitaxel in the plasma sample. According to the plasma sample pretreatment method provided by the invention, the stability of the paclitaxel lipidosome in a repeated freezing and thawing environment is improved, the paclitaxel lipidosome cannot be broken or leaked after repeated freezing and thawing can be ensured, the method is simple and convenient to operate, the test cost is low, long-time continuous operation is not needed, the reasonable working time of operators is ensured, and the working efficiency is improved. Therefore, the accuracy of subsequent experiment detection is ensured.

Description

technical field [0001] The invention relates to a sample processing method, in particular to a plasma sample preprocessing method for improving the freezing and thawing stability of paclitaxel liposomes. Background technique [0002] Paclitaxel is a natural secondary metabolite isolated and purified from the bark of the gymnosperm Taxus chinensis. It has been clinically proven to have a good anti-tumor effect. Balance, induce and promote tubulin polymerization, microtubule assembly, prevent depolymerization, thereby stabilizing microtubules and inhibiting the mitosis of cancer cells and triggering apoptosis, thereby effectively preventing the proliferation of cancer cells, especially for the high incidence of cancer. High ovarian cancer, uterine cancer and breast cancer have special effects. Paclitaxel is the most popular anticancer drug on the international market in recent years, and is considered to be one of the most effective anticancer drugs for mankind in the next 20...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/06G01N30/02
Inventor 蒋丽英王逸帆金晓莺施婧
Owner WUXI APPTEC SUZHOU
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