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Lactobacillus acidophilus LA16 antagonizing helicobacter pylori and application of lactobacillus acidophilus LA16

A technology of Helicobacter pylori and Lactobacillus acidophilus, applied in the field of microorganisms, can solve the problems of drug resistance of patients, achieve high gastric acid-resistant survival rate, good use effect, and convenient storage and portability

Active Publication Date: 2022-08-02
江苏新申奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And long-term use of the common clinical treatment method "antibiotic triple / quadruple" will cause certain drug resistance in patients

Method used

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  • Lactobacillus acidophilus LA16 antagonizing helicobacter pylori and application of lactobacillus acidophilus LA16
  • Lactobacillus acidophilus LA16 antagonizing helicobacter pylori and application of lactobacillus acidophilus LA16
  • Lactobacillus acidophilus LA16 antagonizing helicobacter pylori and application of lactobacillus acidophilus LA16

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening and identification of Lactobacillus acidophilus LA16

[0034] 1. Screening of Lactobacillus acidophilus LA16

[0035] 1.1 Sample source

[0036] The strains used in the present invention are all collected from the fermented goat milk product in the Hulunbuir grassland of Inner Mongolia—self-made yogurt, that is, the self-made yogurt of the present invention from Inner Mongolia is taken as a sample.

[0037]1.2 Preparation of culture medium

[0038] The medium used for sample isolation and strain screening is MRS liquid medium with the following components: peptone 10g, beef powder 5g, yeast powder 4g, dipotassium hydrogen phosphate 2g, triammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, magnesium sulfate 0.2g, manganese sulfate 0.05g, agar 15g, distilled water 1000mL.

[0039] 1.3 Isolation of strains

[0040] Put 1g of the sample into 10mL of the MRS liquid medium prepared in step 1.2, mix well and incubate at 36°C, and then dr...

Embodiment 2

[0053] The preparation of embodiment 2 Lactobacillus acidophilus LA16 powder

[0054] MRS liquid medium: peptone 10g, beef powder 5g, yeast powder 4g, dipotassium hydrogen phosphate 2g, triammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, magnesium sulfate 0.2g, manganese sulfate 0.05g, Agar 15g, distilled water 1000mL.

[0055] Preparation method: take out the glycerol tube containing Lactobacillus acidophilus LA16 from -80 ℃, activate and cultivate, and obtain a bacterial content of 10 8 The bacterial seed liquid of cfu / g was diluted 100 times and then inoculated into MRS liquid medium with an inoculum amount of 3wt%. Under the condition of 36°C, facultative anaerobic culture was carried out for 24h (oxygen concentration 8%, carbon dioxide concentration 7% , the environment with a nitrogen concentration of 85%, the % is volume %), the bacterial liquid is centrifuged and vacuum freeze-dried to obtain Lactobacillus acidophilus LA16 powder, and the number of v...

Embodiment 3

[0056] Example 3 Products for the treatment of gastric ulcer

[0057] Composition of raw materials (parts by weight): 3 parts of Lactobacillus reuteri LR06 powder, 1 part of Bacillus coagulans BC01 powder, 2 parts of Lactobacillus acidophilus LA16 powder prepared in Example 2, 0.3 part of soybean oligosaccharide, lactulose 0.2 part, 0.05 part of alpha-lipoic acid, 0.05 part of lactic acid.

[0058] After mixing each component, stir and mix for 30 minutes to obtain a product for treating gastric ulcer.

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Abstract

The invention provides lactobacillus acidophilus LA16 for antagonizing helicobacter pylori and application of the lactobacillus acidophilus LA16, and belongs to the technical field of microorganisms. The lactobacillus acidophilus LA16 is classified and named as lactobacillus acidophilus, the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.22771, the preservation date is June 24, 2021, the preservation unit is China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the postcode is 100101. The lactobacillus acidophilus LA16 obtained through separation has a high gastric acid resistant survival rate, effectively inhibits colonization and growth activity of helicobacter pylori, has a good curative effect on gastroenteritis, gastric ulcer, duodenal ulcer and the like, and has a good clinical effect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to Lactobacillus acidophilus LA16 which antagonizes Helicobacter pylori and its application. Background technique [0002] Helicobacter pylori (Hp) is a gram-negative bacterium with a spiral curve, polyflagellates, blunt ends, and unipolar. , duodenitis and other major causative factors of gastrointestinal diseases. The key to the treatment of patients with peptic ulcer infected by Helicobacter pylori is to eradicate Helicobacter pylori. Quadruple therapy is a common method for clinical treatment of this disease, which can effectively eradicate Helicobacter pylori infection and improve the condition. However, with the discovery of clinical treatment, due to factors such as drug resistance of Helicobacter pylori and adverse reactions of antibiotics, the eradication rate of Helicobacter pylori has declined, which affects the therapeutic effect. [0003] Lactic acid bacteria i...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61K35/742A61K31/385A61K31/702A61K31/19A61K31/7016A61P1/04A61P31/04C12R1/23C12R1/225C12R1/07
CPCC12N1/20A61K35/747A61K35/742A61K31/385A61K31/702A61K31/19A61K31/7016A61P1/04A61P31/04A61K2300/00Y02A50/30
Inventor 马凯季锋
Owner 江苏新申奥生物科技有限公司
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