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Copper-cobalt bimetal organic framework nano-enzyme and colorimetric analysis method of organic phosphorus colorimetric sensor of copper-cobalt bimetal organic framework nano-enzyme

An organic framework and bimetallic technology, applied in the field of colorimetric analysis, can solve the problems of small activity range and poor anti-interference ability

Pending Publication Date: 2022-08-02
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims at the problems of small activity range and poor anti-interference ability in the colorimetric detection of organophosphorus by using natural enzymes in the prior art, and firstly provides a copper-cobalt bimetallic organic framework nanozyme, using its metal-organic framework composite material Large interface specific surface area, good stability and good catalytic performance to show excellent enzyme-like catalytic performance

Method used

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  • Copper-cobalt bimetal organic framework nano-enzyme and colorimetric analysis method of organic phosphorus colorimetric sensor of copper-cobalt bimetal organic framework nano-enzyme
  • Copper-cobalt bimetal organic framework nano-enzyme and colorimetric analysis method of organic phosphorus colorimetric sensor of copper-cobalt bimetal organic framework nano-enzyme
  • Copper-cobalt bimetal organic framework nano-enzyme and colorimetric analysis method of organic phosphorus colorimetric sensor of copper-cobalt bimetal organic framework nano-enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this example, the copper-cobalt bimetallic organic framework nanozyme was first prepared.

[0029] One-pot synthesis of copper-cobalt bimetallic organic framework nanozymes by soft-template Weigh ultrapure water with a concentration of 0.045 mmol / mL of copper nitrate, put the aforementioned components into reactor A, and ultrasonically mix for 10 min to obtain a pale pink solution A after mixing evenly; then take another reactor B, press 0.79 The concentration of M to prepare solution B of 2-methylimidazole and ultrapure son, and then according to the molar ratio of 2-methylimidazole to copper nitrate trihydrate is 2750:20, add solution A dropwise to reactor B, at room temperature Under rapid stirring for 1 hour, after the reaction, let stand for 15 min, the solution was centrifuged at 8000 rpm, and washed three times with absolute ethanol, and finally at 60 o The CuCo-ZIF nanozyme particles were obtained by vacuum drying under the condition of C for 12 h.

[0030]...

Embodiment 2

[0032]One-pot synthesis of copper-cobalt bimetallic organic framework nanozymes by soft-template Weigh ultrapure water with a concentration of 0.04 mmol / mL of copper nitrate, put the aforementioned components into reactor A, and ultrasonically mix for 10 min to obtain a pale pink solution A after mixing evenly; then take another reactor B, press 0.75 The concentration of M to prepare solution B of 2-methylimidazole and ultrapure son, and then according to the molar ratio of 2-methylimidazole to copper nitrate trihydrate of 2500:25, add solution A dropwise to reactor B, at room temperature Under rapid stirring for 1 hour, after the reaction, let stand for 15 min, the solution was centrifuged at 8000 rpm, and washed three times with absolute ethanol, and finally at 70 o The CuCo-ZIF nanozyme particles of this example were obtained by vacuum drying under the condition of C for 10 h.

Embodiment 3

[0034] In this example, the figure 2 The principle schematic diagram of the organophosphorus colorimetric sensor and colorimetric analysis method of the copper-cobalt bimetallic organic framework nanozyme is shown. The copper-cobalt bimetallic organic framework nanozyme prepared in Example 1 is used as the active ingredient, and the colorimetric of the organic phosphorus colorimetric sensor is carried out. The study of analytical methods.

[0035] In this example, 1 mg of the prepared copper-cobalt bimetallic organic framework nanozyme was ultrasonically dispersed in 1 mL of deionized water to obtain a uniform dispersion; then, 0.2 mL of the dispersion was added to a mixture of 0.1 M Na 2 HPO 4 and NaH 2 PO 4 PBS phosphate buffer solution composed of PBS, where the pH value of PBS is 4, add a known concentration of 0.1 mL of methyl pyrimidin (organophosphorus) solution, shake and disperse to obtain a uniform mixture; finally, add 0.2 mL of 20 mM TMB solution and 0.02 mL o...

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Abstract

The invention relates to a copper-cobalt bimetal organic framework nano-enzyme and a colorimetric analysis method of an organic phosphorus colorimetric sensor thereof, which comprises the following steps: firstly, synthesizing the copper-cobalt bimetal organic framework nano-enzyme (CuCo-ZIF) by taking CTAB (Cetyltrimethyl Ammonium Bromide) as a soft template, 2-methylimidazole as an organic ligand, copper nitrate trihydrate as a copper source, cobalt nitrate hexahydrate as a cobalt source and deionized water as a solvent; the CuCo-ZIF nano-enzyme synthesized by the method has a regular cubic structure, so that the CuCo-ZIF nano-enzyme has a relatively large specific surface area, high porosity and high stability, and the peroxidase activity of the nano-enzyme is greatly improved. The method comprises the following steps: firstly, preparing a nano-enzyme, then constructing a colorimetric sensor by using the nano-enzyme, performing colorimetric analysis to directly and visually detect organophosphorus, can quickly, simply and visually realize high-sensitivity detection of organophosphorus, and has the advantages of good stability, low detection limit, high sensitivity, good specificity and the like.

Description

technical field [0001] The invention relates to the technical field of colorimetric analysis, in particular to a colorimetric analysis method for a copper-cobalt bimetallic organic framework nanozyme and an organic phosphorus colorimetric sensor. Background technique [0002] Organophosphorus pesticides, as one of the most common pesticides, are widely used in many scenarios such as agriculture, animal husbandry and public gardens. At the same time, the abuse of organophosphorus pesticides on many occasions has also caused pollution to water resources, fruits, vegetables and processed foods, which has caused great damage to the ecological environment and human health. Therefore, the efficient and sensitive detection of organophosphorus content in the environment is particularly important. Colorimetric sensors have attracted great interest in the detection of organophosphorus concentrations in samples due to their high sensitivity, fast assay speed, high stability, and low c...

Claims

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Application Information

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IPC IPC(8): C08G83/00B01J31/22G01N21/31G01N21/78
CPCC08G83/008B01J31/1691B01J31/1815B01J37/0018G01N21/78G01N21/31B01J35/40Y02A50/20
Inventor 杨占军肖嘉祥石凤李娟
Owner YANGZHOU UNIV
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