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Protein WG-SP01 with immunomodulatory activity

A technology of immunomodulatory activity and immunomodulatory agent, applied in the field of protein engineering, can solve problems such as adverse effects on immune function, and achieve the effect of improving ATP activity and increasing proliferation ability

Pending Publication Date: 2022-07-29
HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, many experts have been studying other treatment methods, and it has been found that certain biological agents and natural medicines suppress autoimmune responses, but have little or no adverse effects on the immune function against infection and tumor
At present, there is an urgent need for an effective food or medicine for the treatment of immune diseases

Method used

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  • Protein WG-SP01 with immunomodulatory activity
  • Protein WG-SP01 with immunomodulatory activity
  • Protein WG-SP01 with immunomodulatory activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation, screening and identification of protein WG-SP01 with immunomodulatory activity.

[0028] Using wheat germ as raw material, after enzymatic hydrolysis by alkaline protease, centrifugation, ultrafiltration, separation and purification, screening and identification, the protein WG-SP01 with immunomodulatory activity was obtained.

[0029] The specific method is as follows: mix wheat germ and 3% NaCl solution according to the ratio of 1:10, add alkaline protein (2000U / g), and the amount added is alkaline protease: protein mass ratio in the sample=1:200, at 55 ℃ , under the condition of pH9.0, enzymatic hydrolysis for 0.5h, NaOH adjusting pH7.0 to complete the reaction, then centrifuging at 5000rpm for 10min, taking the supernatant, the supernatant was separated by membrane filtration and column chromatography, and the in vitro lymphocyte proliferation experiment was carried out. The immunomodulatory active protein WG-SP01 was obtained by screening, an...

Embodiment 2

[0031] Example 2 Detection of immunomodulatory activity of protein WG-SP01.

[0032] 1. Determination of immune activity in vitro - mouse spleen lymphocyte proliferation assay

[0033] (1) Preparation of spleen cell suspension

[0034] Balb / c mice aged 6-8 weeks were sacrificed by decapitation, immersed in 75% ethanol, and then placed on an ultra-clean workbench for aseptic laparotomy. The spleen was taken out and placed on a 200-mesh cell sieve above a culture dish containing Hank's solution. Then wash with Hank's solution, squeeze and grind with a disposable glass syringe core, wash twice with Hank's solution, transfer to a centrifuge tube to make a suspension, centrifuge at 1000rpm for 5-10min, and discard the supernatant. Take 6 mL of cell lysate that has been autoclaved or filtered with a 0.22 μm filter, add to a centrifuge tube to lyse red blood cells, blow evenly, add Hank’s solution after 1-2 min, centrifuge at 1000 rpm for 5-10 min, and discard the supernatant. Add ...

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Abstract

The invention discloses an immunomodulatory active protein WG-SP01, and the protein WG-SP01 can activate the activity of LDH and ACP, so that macrophages of an organism are in an activated state; the ATP activity of lymphocytes can be obviously improved; the multiplication capacity of T lymphocytes and B lymphocytes of the spleen is obviously improved; the protein WG-SP01 has a remarkable immune regulation effect. Therefore, the protein WG-SP01 can be used as an active ingredient of an immunomodulator or an immunopotentiator for preparing medicines or functional foods for treating immune diseases such as systemic lupus erythematosus, rheumatoid arthritis, scleroderma and hyperthyroidism, and also can be used for other pharmaceuticals or functional foods with an immunoregulation effect.

Description

technical field [0001] The invention relates to the field of protein engineering, in particular to a protein WG-SP01 with immunomodulatory activity. Background technique [0002] Immune diseases refer to diseases caused by the imbalance of immune regulation that affects the immune response of the body. Autoimmune diseases are mainly caused by the accumulation of a large number of immune cells and immunoglobulins produced by an excessive immune response, which damages normal tissues and causes lesions and functional impairments. If it occurs in the skin, it is allergic dermatitis and various skin lesions. If it occurs in the joints, it is arthritis. If it occurs in the kidneys, it is nephritis. [0003] Common autoimmune diseases are: systemic lupus erythematosus, rheumatoid arthritis, scleroderma, hyperthyroidism, juvenile diabetes, idiopathic thrombocytopenic purpura, autoimmune hemolytic anemia, ulcerative colitis, and many disease, chronic liver disease...etc. [0004]...

Claims

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Application Information

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IPC IPC(8): C07K14/415A23L33/18A61P37/02
CPCC07K14/415A23L33/18A61K38/16A61P37/02A61K38/00
Inventor 侯银臣黄继红廖爱美潘龙魏兆军董得平杨盛茹连伟帅李琰袁晓晴
Owner HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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