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Trichoderma harzianum and application thereof as well as anthracnose-resistant microbial agent for promoting growth and development of strawberries

A technology of Trichoderma harzianum and microbial inoculum, which is applied in the direction of microorganisms, biocides, plant growth regulators, etc., can solve the problems of difficult food safety, reduced fertilizer utilization rate, and reduced strawberry quality, so as to improve soil supply. Nitrogen levels, enhanced growth, photosynthetic effects

Active Publication Date: 2022-07-22
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, strawberry production management in our province has always had an outdated concept of focusing on yield and neglecting quality. The continuous cropping of soil and the high-intensity and high-frequency input of chemical fertilizers and pesticides have resulted in the accumulation of pathogenic bacteria in strawberry planting sheds, chemical pesticide residues, and soil salinization and acidification. , the fertilizer utilization rate is reduced, resulting in a decline in the quality of strawberries, and it is difficult to guarantee food safety

Method used

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  • Trichoderma harzianum and application thereof as well as anthracnose-resistant microbial agent for promoting growth and development of strawberries
  • Trichoderma harzianum and application thereof as well as anthracnose-resistant microbial agent for promoting growth and development of strawberries
  • Trichoderma harzianum and application thereof as well as anthracnose-resistant microbial agent for promoting growth and development of strawberries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation and identification of strains

[0030] Separation process: During the strawberry growing season, the soil of healthy strawberries was collected from Jurong, Jiangsu, and then packed in sterile plastic bags and brought back to the laboratory, and stored in a refrigerator at 4°C for later use. Weigh 10g of healthy strawberry rhizosphere soil, put it in a 250mL conical flask, then add 100mL of sterile water, shake to obtain a rhizosphere soil suspension; draw 100μL of 10 -4 -10 -6 Soil suspensions of different dilutions were evenly spread on beef extract peptone, PDA, Gao's No. 1 medium plate, 2-3 parallels were made for each dilution, and then placed in a constant temperature incubator at 28°C for 3- 5d. When the colony grows, pick a single colony and move it into the corresponding medium for purification, and describe the colony morphology, pick a single colony, make slices, microscopically examine, take pictures, and finally transfer the purified ...

Embodiment 2

[0034] Example 2: Preparation of microbial fermentation broth

[0035] (1) Preparation of seed solution: inoculate Trichoderma harzianum CM-26 into the activation medium, and cultivate at 35°C for 28 hours to obtain Trichoderma harzianum CM-26 activated strain; wherein, the composition of the activation medium is: peptone 1.0g / L, yeast powder 1.5g / L, sodium chloride 0.2g / L, pH 7.2.

[0036] (2) Preparation of fermentation broth: inoculate the activated strain with a volume ratio of 5.0% into the fermentation medium, and cultivate at 35°C for 60 hours to obtain Trichoderma harzianum CM-26 fermentation broth; wherein, the composition of the fermentation medium is: glucose 3.5g / L, peptone 1.5g / L, yeast extract 1.0g / L, potassium dihydrogen phosphate 0.5g / L and calcium carbonate 0.6g / L, pH 7.2.

[0037] The technical indicators of the fermentation broth are: the number of effective viable bacteria ≥ 2.5 billion / mL.

Embodiment 3

[0038] Example 3: Potassium alginate-containing microbial inoculum

[0039] After the potassium alginate is pulverized, pass through a 60-mesh sieve to obtain potassium alginate powder, and then bottled with the microbial fermentation broth containing Trichoderma harzianum CM-26 prepared in the above-mentioned embodiment 2 according to the mass ratio of 10:40, and then bottled at 25 Store at ℃ to obtain potassium alginate-containing microbial inoculum, CM-26 Trichoderma harzianum effective viable bacterial count ≥ 2 billion / mL, and pH value of 5.0 to 8.0.

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Abstract

The invention discloses trichoderma harzianum, application thereof and a microbial agent capable of resisting anthracnose and promoting growth and development of strawberries. The invention discloses trichoderma harzianum CM-26, which is classified and named as trichoderma harzianum, and the preservation number of the trichoderma harzianum CM-26 is CGMCC (China General Microbiological Culture Collection Center) NO.40010. The microbial agent comprises a fermentation broth of the trichoderma harzianum, and can further comprise potassium alginate or / and a raspberry leaf extract. The microbial agent prepared from the trichoderma harzianum has remarkable effects in the aspects of promoting strawberry growth, promoting photosynthesis and preventing and controlling strawberry anthracnose.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a Trichoderma harzianum and its application and a microbial inoculum for resisting anthracnose and promoting the growth and development of strawberries. Background technique [0002] Strawberry is an important economic crop widely cultivated in Jiangsu Province, and its cultivation area and output rank among the top in the country. For a long time, the strawberry production management in our province has always been an outdated concept of focusing on yield and light quality. Continuous soil cropping and high-intensity and high-frequency input of chemical fertilizers and pesticides have resulted in the accumulation of pathogenic bacteria in strawberry planting sheds, chemical pesticide residues, soil salinization and acidification , the fertilizer utilization rate is reduced, resulting in a decline in the quality of strawberries, and food safety is difficult to guarantee. Str...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14A01N63/38Y02A40/10
Inventor 王晓琳吉沐祥黄洁雪邬劼
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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