Novel coronavirus vaccine with mumps virus as live vector

A mumps virus, coronavirus technology, applied in the direction of antiviral agents, introduction of foreign genetic material using vectors, antibody medical components, etc. The effect of preventing mumps virus

Active Publication Date: 2022-07-22
BEIJING CELL FUSION BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no combined vaccine against both SARS-CoV-2 and mumps virus, and the number of vaccinations against SARS-CoV-2 and mumps virus is very large

Method used

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  • Novel coronavirus vaccine with mumps virus as live vector
  • Novel coronavirus vaccine with mumps virus as live vector
  • Novel coronavirus vaccine with mumps virus as live vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: a kind of novel coronavirus vaccine with mumps virus as live carrier

[0042] This example provides a new coronavirus vaccine using mumps virus as a live vector, and the vaccine uses the mumps virus attenuated strain QS-F46 (the mumps virus attenuated strain QS-F46 is the mumps virus strain QS -F pass 46 generations attenuated income, the deposit number of mumps virus strain QS-F is CCTCC No: V202182, which is recorded in the patent application text of publication number CN111019910A, the deposit number of mumps virus attenuated strain QS-F46 CCTCC No: V202182) is a live vector expressing the RBD protein of the new coronavirus (the amino acid sequence is shown in SEQ ID NO.5, and the nucleotide sequence is shown in SEQ ID NO.6), wherein the RBD protein encoding the RBD protein is The gene is inserted between the NP segment and the P / V segment of the mumps virus genome (see figure 1 ).

[0043] The preparation method of the vaccine comprises the following...

experiment example 1

[0054] Experimental Example 1: Performance Verification of Recombinant Virus QS-F-RBD (SARS-CoV-2)

[0055] Experiment 1: Vero cells (purchased from ATCC) were grown at 8 × 10 5 The inoculum amount of each cell was plated in advance into a six-well plate containing 2000 μL / well DMEM medium (purchased from Gibco), and cultured at 37°C until the cells became monolayer; The attenuated inflammatory virus strain QS-F46) was used as a control, and the recombinant virus QS-F-RBD (SARS-CoV-2) prepared in Example 1 was inoculated into Vero cells that grew into monolayers at an inoculum of 0.01MOI (6. well cell culture plate), after adsorption and infection at 37 °C for 1 h, the DMEM medium was removed with a pipette, and 2% (v / v) fetal bovine was added to the six-well plate at an additive amount of 2000 μL / well. Serum cell maintenance solution (the cell maintenance solution is DMEM medium purchased from Gibco Company), cultured at 34°C, and the culture supernatant was collected on 1d,...

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Abstract

The invention relates to a novel coronavirus vaccine taking mumps virus as a live vector, and belongs to the technical field of biological medicines. The invention provides a novel coronavirus vaccine taking a mumps virus as a live vector. The vaccine takes the mumps virus as the live vector to express glycoprotein on the surface of the novel coronavirus, a functional subunit of the glycoprotein on the surface of the novel coronavirus or a receptor protein of the functional subunit of the glycoprotein on the surface of the novel coronavirus. The vaccine can be used as a combined vaccine, and can be used for preventing mumps virus and SARS-CoV-2 at the same time.

Description

technical field [0001] The invention relates to a novel coronavirus vaccine using mumps virus as a live carrier, and belongs to the technical field of biomedicine. Background technique [0002] The new coronavirus SARS-CoV-2 is the seventh known coronavirus that can infect humans. Compared with SARS virus and epidemic influenza virus, SARS-CoV-2 has a faster human-to-human transmission rate (R0=2.5), a longer incubation period (4-12), and the high viral load in the upper respiratory tract causes patients to have a Highly contagious. The clinical symptoms are mainly fever, dry cough, and fatigue. Severe cases often have dyspnea, which rapidly progresses to acute respiratory distress syndrome, septic shock, and even metabolic acidosis and coagulation dysfunction, which seriously endangers human health. At this stage, there is no specific drug for the new coronavirus, and vaccine research and development is in full swing around the world, mainly focusing on inactivated vaccin...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61P31/14C12N15/86C12N15/50
CPCA61K39/12A61P31/14C12N15/86C07K14/005C12N2770/20034A61K2039/5256C12N2760/18743C12N2770/20022
Inventor 安祺田大勇李先凤张亚静
Owner BEIJING CELL FUSION BIOTECHNOLOGY CO LTD
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