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Application of polypeptide receptor PSKR1 gene in improving high-temperature adversity resistance of tomato plant and/or tomato pollen

A gene and tomato technology, applied in the biological field, can solve the problems of tomato's poor heat resistance and achieve the effect of improving resistance and high temperature stress resistance

Active Publication Date: 2022-07-15
安庆市长三角未来产业研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The Chinese patent document with the publication number CN108841841A discloses the cloning of a tomato transcription factor SlbZIP6 and its application in high temperature stress resistance. wild type worse

Method used

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  • Application of polypeptide receptor PSKR1 gene in improving high-temperature adversity resistance of tomato plant and/or tomato pollen
  • Application of polypeptide receptor PSKR1 gene in improving high-temperature adversity resistance of tomato plant and/or tomato pollen
  • Application of polypeptide receptor PSKR1 gene in improving high-temperature adversity resistance of tomato plant and/or tomato pollen

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Construction and identification of PSKR1 gene overexpression vector and tomato plants

[0035] The coding sequence of the PSKR1 gene (Solyc01g008140) was obtained according to the tomato genome database (https: / / solgenomics.net / ). The nucleotide sequence of the protein coding region is shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by the PSKR1 gene is shown in shown in SEQ ID NO.2. Referring to the Gateway manual, Primer5 software was used to design the upstream primer (SEQ ID NO.3): 5'>TTggcgcgccATGGTGATTTGGGAGTTTCT>3' and the downstream primer (SEQ ID NO.4) 5'>CGGggtaccCCTCTCCTTTACACTTGCGATTG>3'.

[0036] RNA was extracted from tomato leaves, and cDNA was obtained by reverse transcription. Using the cDNA as a template, the full-length coding region of the tomato PSKR1 gene was amplified by PCR using the upstream and downstream primers. On the vector pFGC1008-3HA, the expression vector pFGC1008-3HA is specifically: three HA-tagged proteins a...

Embodiment 2

[0038] Study on high temperature stress resistance of plants overexpressing PSKR1 gene

[0039] Place 3-4 week old tomato seedlings in an artificial climate incubator with 12 hours of light and 12 hours of darkness, light intensity 200 μmol m -2 s -1 , the temperature is set to the high temperature treatment of 45 ℃ or the normal temperature treatment of 25 ℃, during which water is added in time to prevent the seedlings from drying out. Two days later, it was found that the wild-type control plant WT wilted more seriously than the PSKR1 gene overexpressing plants OE:PSKR1-1 and OE:PSKR1-2 under high temperature conditions. Representative plants were selected to take pictures, and the maximum photochemical efficiency (Fv) of PSII in tomato plants was determined. / Fm) and relative conductivity.

[0040] Among them, the maximum photochemical efficiency (Fv / Fm) of plant PSII was determined as follows: after tomato plants treated at different temperatures were placed in a dark en...

Embodiment 3

[0044] Effects of exogenous treatment of plant polypeptide PSK on high temperature stress resistance of plants

[0045] Slowly add 0.85g of PSK to 100mL of water, stir until fully dissolved to obtain a mixed solution; add 9.9L of water and 2.5mL of silicone to the 100mL mixed solution and mix to form a diluted solution (preparation working solution) before use.

[0046] The preparation working solution was evenly sprayed on the surface of the tomato leaves with five leaves and one heart, until the leaves on both sides were completely wet, and the preparation working solution did not flow down in the form of water droplets. tomato plants served as controls.

[0047] Different temperature treatments were performed on tomato plants after exogenous spraying of PSK and control treatments. Tomato plants were placed in an artificial climate incubator with a light intensity of 200 μmol m -2 s -1 , the temperature is set to 45 ℃ high temperature treatment or 25 ℃ normal temperature ...

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Abstract

The invention discloses application of a PSKR1 gene or a protein coded by the PSKR1 gene in improving high-temperature stress resistance of a tomato plant and / or tomato pollen, a nucleotide sequence of a protein coding region of the PSKR1 gene is shown as SEQ ID NO.1, and an amino acid sequence of the protein is shown as SEQ ID NO.2. The invention further discloses application of the PSKR1 gene or the protein coded by the PSKR1 gene in improving high-temperature stress resistance of the tomato plant and / or the tomato pollen. The PSKR1 gene can interact with HsfA1a to regulate and control the resistance of tomato plants and / or tomato pollen to high-temperature stress, a PSKR1 gene overexpression vector is constructed through a transgenic technology, and PSKR1 gene overexpression homozygous strain OE: PSKR1-1 and OE: PSKR1-2 transgenic tomatoes obtained through screening by utilizing a tissue culture technology have obvious high-temperature stress resistance compared with a wild type control group, so that the high-temperature stress resistance of tomato plants and / or tomato pollen can be regulated and controlled. It is proved that overexpression of the PSKR1 gene can improve the resistance of tomato plants and / or tomato pollen to high-temperature stress, and important gene resources are provided for cultivation of high-temperature-resistant tomato varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a polypeptide receptor PSKR1 gene in improving the resistance of tomato plants and / or tomato pollen to high temperature stress. Background technique [0002] Tomato (Solanum lycopersicum L.) is one of the most widely cultivated vegetables and cash crops in the world. Because of its delicious taste, rich in vitamins, minerals and carotenoids, it has high nutritional and health care value and is widely loved by consumers. Tomato is a heat-loving and heat-labile crop, and the most suitable growth temperature is 20-25 °C. However, with the global warming, the increase of the greenhouse effect and the poor controllability of the cultivation environment, tomato crops in summer production usually suffer from high temperature. infringe. Tomato is very sensitive to high temperature. High temperature during vegetative growth will significantly inhibit photosynthesis and in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84C07K14/415C12N15/29A01H6/82A01H5/00
CPCC12N15/8271C12N15/8205C07K14/415Y02A40/146
Inventor 师恺王萍罗倩方瀚墨
Owner 安庆市长三角未来产业研究院
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