Method for preparing plant-based fat cultured meat

Abstract

In order to overcome the defects of conventional scaffolds for adipogenic stem cell culture and the defects of flavor, nutrition, taste, juiciness and the like of existing cultured meat, the invention provides application of plant drawing protein in an adipogenic stem cell in-vitro culture scaffold and a preparation method of plant-based fat cultured meat. The method specifically comprises the following steps: S1, pretreatment of a plant wiredrawing protein scaffold: after plant wiredrawing protein absorbs water and expands, cutting the plant wiredrawing protein into appropriate sizes, drying, sterilizing, and soaking the plant wiredrawing protein scaffold in a growth culture medium overnight under an aseptic condition; s2, adding the adipogenic stem cells into a growth culture medium to prepare a cell suspension, inoculating the cell suspension onto a plant drawing protein scaffold, and carrying out multiplication culture; and S3, carrying out proliferation culture until the coverage rate of cells on the scaffold reaches 80-90%, carrying out induced differentiation culture until the cells are differentiated into fat cells, and finally preparing the plant-based fat culture meat with the animal meat flavor.

Description

technical field [0001] The invention belongs to the technical field of stem cell and animal cell cultured meat, and in particular relates to a preparation method of vegetable-based fat cultured meat. Background technique [0002] Meat is rich in protein and micronutrients and has been widely consumed by humans due to easy access to energy, high-quality protein, palatability, and energy. As the world's population grows and becomes more affluent, meat consumption increases year by year. Traditional meat production relies on animal husbandry and not only carries risks of animal diseases, epidemics and antibiotic abuse, but also negatively impacts animal welfare, resources and the environment, and even threatens public health such as ischaemic heart disease and colorectal cancer. Therefore, in order to achieve sustainable meat production, it is urgent to develop a green and efficient way of meat production to partially replace traditional meat production. [0003] Cultured me...

AI Technical Summary

Problems solved by technology

[0007] At present, the conventional scaffolds for adipogenic stem cell culture are: animal-derived scaffolds such as collagen scaffolds, and the original intention of cultured meat is to reduce or avoid the use of animal-derived materials. Therefore, animal-derived scaffolds are contrary to the original intention of cultured meat; another example is cellulose scaffolds. Such as plant-derived scaffolds, the cell adhesion effect is poor, and the culture effect is not good

Moreover, the existing conventional scaffolds need to be cross-linked with chemical reagents when inoculating cells. Most of the cross-linking agents are inedible due to safety issues and are expensive, which seriously restricts the promotion of cultured meat.

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