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Application of long-chain non-coding RNA ST8SIA6-AS1 as pituitary tumor molecular marker

An RNAST8SIA6-AS1, long non-coding technology, applied in the field of tumor molecular biology, can solve the problem that there is no report of long non-coding RNAST8SIA6-AS1 associated with invasive pituitary tumors.

Inactive Publication Date: 2022-07-08
李作伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Long non-coding RNA ST8SIA6-AS1 associated with aggressive pituitary tumors has not been reported in the prior art

Method used

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  • Application of long-chain non-coding RNA ST8SIA6-AS1 as pituitary tumor molecular marker
  • Application of long-chain non-coding RNA ST8SIA6-AS1 as pituitary tumor molecular marker
  • Application of long-chain non-coding RNA ST8SIA6-AS1 as pituitary tumor molecular marker

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0048] The expression levels of ST8SIA6-AS1 and HOXA9 in non-invasive pituitary adenomas and invasive pituitary adenomas were detected by qRT-PCR. The specific steps are as follows:

[0049] 1.1 Primer design: The sequence of the long-chain non-coding RNA ST8SIA6-AS1 was found in the gene library NCBI as shown in SEQID NO.1, the gene ID of HOXA9 is 3205, and the transcript is NM_152739.4. Primer software is used to design the primers as follows:

[0050] The primers for ST8SIA6-AS1 are as follows:

[0051] Upstream primer: 5'-TTCAGTGGCATGGTTCAGTC-3'SEQ ID NO.2;

[0052] Downstream primer: 5'-CTCAGAGGCGACAGGGGTTTC-3'SEQ ID NO.3;

[0053] The primers for HOXA9 are as follows:

[0054] Upstream primer: 5'-ATGCTTGTGGTTCTCCTCCA-3'SEQ ID NO.4;

[0055] Downstream primer: 5'-AGTTGGCTGCTGGGTTATTG-3'SEQ ID NO.5.

[0056] 1.2 Clinical sample collection: 30 non-invasive pituitary adenomas and 30 invasive pituitary adenomas diagnosed in Shandong Provincial Hospital of Traditional Chin...

experiment example 2

[0064] To detect the effect of ST8SIA6-AS1 on the proliferation, invasion and migration of GH3 and GTI-1 pituitary adenoma cells, the specific steps are as follows:

[0065] 2.1 Cell culture: Both GH3 and GTI-1 cells were cultured in complete medium containing 10% fetal bovine serum by volume. at 37°C, 5% CO 2 cultured in an incubator. After 80% of cells were confluent, cells were trypsinized with 0.25%. Subculture was performed at a ratio of 1:3.

[0066] 2.2 Cell transfection: adjust the number of cells to 2 × 10 8 / mL, inoculated into the corresponding culture plate after counting. GH3 and GTI-1 cells were plated at 1 × 10 per well 5 The density of cells was seeded into 6-well plates. After a period of incubation, the medium was replaced with serum-free medium (Ham's F-12K medium). Continue to incubate for 24 hours. Add 10 μL of pcDNA3.1-ST8SIA6-AS1 or sh-ST8SIA6-AS1 and control pcDNA3.1-NC or sh-NC to 50 μL of serum-free medium. Mix gently and let stand for 5 minu...

experiment example 3

[0073] qRT-PCR was used to detect the effect on the EMT ability of cells, and the specific steps were as follows:

[0074] 3.1 PCR: The experimental method is the same as above.

[0075] The control group was the tumor cell group without ST8SIA6-AS1 knockdown, and the experimental group was the ST8SIA6-AS1 knockdown group.

[0076] 3.2 Experimental results: see image 3 , In GH3 and GTI-1 cells, the expression of E-cadherin increased in sh-ST8SIA6-AS1 group, while the expression of Vimentin, N-cadherin, SNAIL1, SLUG, β-catenin and TWSIT1 decreased.

[0077] The experimental results showed that: ST8SIA6-AS1 was significantly positively correlated with the expression of cell invasion-related genes.

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Abstract

The invention provides application of long-chain non-coding RNA ST8SIA6-AS1 as a pituitary tumor molecular marker, belongs to the technical field of tumor molecular biology, and particularly relates to application of the long-chain non-coding RNA ST8SIA6-AS1 as the pituitary tumor molecular marker. The application comprises the application of the detection reagent of the long-chain non-coding RNA ST8SIA6-AS1 in preparation of a pituitary tumor diagnosis and prognosis detection kit. The invention further discloses application of the inhibitor of the long-chain non-coding RNA ST8SIA6-AS1 or the inhibitor of the long-chain non-coding RNA ST8SIA6-AS1 in preparation of medicines for treating Wherein the nucleotide sequence of the long-chain non-coding RNA ST8SIA6-AS1 is as shown in SEQ ID NO.1. According to the invention, a potential therapeutic target is provided for pituitary tumors.

Description

technical field [0001] The invention belongs to the technical field of tumor molecular biology, in particular to the application of a long-chain non-coding RNA ST8SIA6-AS1 as a molecular marker for pituitary tumors. Background technique [0002] Pituitary adenomas are common intracranial tumors with diverse biological behaviors due to their cellular proliferative and endocrine properties. Although the malignancy of pituitary adenomas is rare, some pituitary adenomas can infiltrate the surrounding tissues of the sellar region, destroy the normal structure, infiltrate the vascular wall, and invade the cavernous sinus and surrounding brain tissue. The occurrence of pituitary adenomas may be related to the regulation of cell cycle, the expression of oncogenes and the deletion of tumor suppressor genes. But its pathogenesis is still unclear. [0003] Epithelial-mesenchymal transition (EMT) is characterized by a decrease in endothelial adhesion factors and an increase in cytoske...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/118C12Q2600/178C12Q2563/107C12Q2531/113C12Q2525/301C12Q2525/30
Inventor 李作伟
Owner 李作伟
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