Primer, kit and recognition method for human ApoE/SLCO1B1 gene locus

Abstract

The invention discloses a primer, a kit and a recognition method for a human ApoE / SLCO1B1 gene locus. The primer comprises an ApoE gene (c.388Tgt; the invention relates to a wild-type primer of an ApoE gene (c.526Cgt; C) site, the invention relates to a wild type primer of an ApoE gene (c.388Tgt; the invention relates to a mutant primer of an ApoE gene (c.526Cgt, C) site, and an ApoE gene (c.526Cgt; according to the present invention, by designing the amplification primers of different sites of the APOE gene, whether the genes of different sites belong to the wild type or the mutant type can be accurately classified, and the primer of the SLCO1B1 gene site is further designed so as to ensure that the primer can efficiently identify the site types of different genes with high quality; and more accurate genotype data can be given.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis (IVD), in particular to a primer, a kit and an identification method for human ApoE / SLCO1B1 gene locus. Background technique [0002] Statins are drugs that lower low-density lipoprotein cholesterol (LDL-C) levels, thereby reducing the risk of cardiovascular disease. However, different individuals respond differently to statins, and statins are metabolized and transported differently in the liver due to individual genetic characteristics, especially the key transporters involved in statin metabolism in the liver such as organic anion transporting polypeptide (OATP1B1) ( Gene polymorphisms (encoded by SLCO1B1 gene) and apolipoprotein E (ApoE) can affect the plasma and liver concentrations of statins, thereby affecting the efficacy and safety of statins. [0003] Apolipoprotein E is involved in the transport, storage and excretion of blood lipids. The human ApoE gene is located on chrom...

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: (1) Before the PCR reaction, DNA needs to be extracted from the sample to detect the quality and concentration, the operation is complicated, and there is a risk of confusing the sample

(2) When detecting multiple sites, one sample needs to perform multiple reactions, the detection cost is high, the demand for the amount of template is high, the operation intensity is high, and it is easy to cause operation errors and pollution

It can perform high-throughput detection, but this method is expensive, requires a large amount of templates, and the operation and data analysis are complex and time-consuming

[0009] RFLP analysis method: Based on the change of the restriction endonuclease recognition site caused by gene mutation, such as the loss of the site or the generation of a new site, a specific fragment is amplified by PCR, and then the restriction endonuclease is used for the digestion reaction , to observe the size of the fragments by electrophoresis, but it has limitations of enzyme cutting sites, and it is difficult to perform high-throughput parallel analysis

Images