Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nucleic acid detection card box capable of preventing nucleic acid pollution

A detection card, anti-nucleic acid technology, applied in the field of nucleic acid detection to avoid pollution

Pending Publication Date: 2022-07-08
DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve at least one of the above-mentioned problems existing in existing nucleic acid detection devices. To this end, the present invention provides a nucleic acid detection cartridge for preventing nucleic acid pollution and a chip with a detection sample used in conjunction with detection, which is suitable for nucleic acid End-point analysis of the detected product, the use process is completely closed and pollution-free, high reliability, low preparation cost and high fault tolerance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleic acid detection card box capable of preventing nucleic acid pollution
  • Nucleic acid detection card box capable of preventing nucleic acid pollution
  • Nucleic acid detection card box capable of preventing nucleic acid pollution

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0069] The preparation of the nucleic acid detection cartridge for preventing nucleic acid pollution: the first bottom plate A, the second bottom plate B and the cover plate C are respectively made by common processing means such as machine tool and injection molding; The second bottom plate B and the cover plate C are sealed by common sealing means such as hot pressing, laser, ultrasonic, etc.; One side; the test strip 5 is fixedly connected to the side of the second bottom plate B away from the first bottom plate A by means of gluing or the like.

[0070] The use process of the nucleic acid detection cartridge for preventing nucleic acid pollution:

[0071] S1: Sample release: insert the chip containing the test sample into the chip socket, use the chip destruction structure to destroy the sealing film on the chip, and release the test sample to the sample buffer chamber;

[0072] S2: Buffer release: squeeze the liquid storage blister, use the blister release module to dest...

Embodiment 1

[0078]The parameters of this example: including a first base plate with a thickness of 4mm, a second base plate with a thickness of 3mm and a cover sheet with a thickness of 1mm. ; After processing, the first bottom plate, the second bottom plate and the cover sheet were soaked and rinsed with clean water for 30 minutes, then 100 mM NaOH and 0.1% sodium dodecyl sulfate (SDS) ultrasonic bath for 30 minutes, and finally washed with deionized water; The treated first bottom plate and the second bottom plate are covered, and a flexible sealing sleeve is placed at the chip socket, and the two bottom plates are sealed by ultrasonic welding, and then the test strip is fixed on the test paper of the second bottom plate with double-sided tape. Then seal the cover sheet on the side of the second base plate away from the first base plate by ultrasonic welding, and use double-sided tape to fix the liquid storage blister with 100 μL of buffer solution on the first base plate to form a compl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of nucleic acid detection, and discloses a nucleic acid detection card box capable of preventing nucleic acid pollution, which comprises a closed bottom plate, and the bottom plate is provided with a chip socket for inserting and detecting a chip with a detection sample; the chip destroying structure is used for destroying the sealing film on the chip and releasing the detection sample; the liquid storage bubble cap is used for pre-storing a buffer solution; the bubble cap release module is used for damaging the liquid storage bubble cap and releasing the buffer solution; a detection sample and the buffer solution flow into the test strip after being uniformly mixed. According to the nucleic acid detection card box, sealing of the chip sealing film is destroyed through the chip destroying structure, a detection sample is released, a buffer solution is released through the pressure liquid storage bubble cap, the detection sample diluted by the buffer solution automatically flows to a sample loading area of the test strip, and then detection and analysis of a nucleic acid amplification product are achieved on the test strip through chromatography and an immune binding principle.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a universal nucleic acid detection cartridge for preventing nucleic acid pollution. Background technique [0002] Nucleic acid detection is one of the main detection methods in the field of in vitro diagnosis. It is a detection method with high accuracy. Using PCR technology, nucleic acid sequencing technology, molecular hybridization and other technologies, it can quickly detect specific nucleic acid sequences in patient biological samples. , to provide medical test evidence for the diagnosis of infection cases, to win the best treatment time for the infected, and to reduce the virus death rate. It has been widely used in research in various fields such as the diagnosis of infectious diseases, the investigation of epidemic diseases, food hygiene inspection, early diagnosis of tumors and genetic diseases, and forensic identification. [0003] The polymerase chain reaction (...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6804C12Q1/6844
CPCC12Q1/6804C12Q1/6844C12Q2527/125C12Q2565/625
Inventor 王晶何清聪周侗刘仁源陈立勇
Owner DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com