Serum metabolism marker related to pulmonary tuberculosis and application thereof

A technology for metabolic markers and pulmonary tuberculosis, which is applied in the field of serum metabolic markers, can solve the problems of inaccurate distinction, high false positives, and restrictions on wide application, and achieve the effect of simple and convenient use, non-destructive samples, and improved detection efficiency

Pending Publication Date: 2022-07-01
广东省结核病控制中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, although microscopic examination of sputum smear staining is simple and easy, in most cases, Mycobacterium tuberculosis may not be found in the sputum of tuberculosis patients, so the detection rate of microscopic examination is low
In addition, the liquid culture method can slightly increase the positive rate, but it takes too long (2-6 weeks) and requires a specialized biosafety level 3 laboratory
In terms of immunological diagnosis, the Mycobacterium tuberculosis pure protein derivative (PPD) used in the Mycobacterium tuberculosis skin test (TST) contains many mycobacterial species (including pathogenic mycobacteria, environmental mycobacteria Therefore, the specificity of PPD diagnosis of tuberculosis is poor, and it is impossible to accurately distinguish whether a positive PPD test result is due to BCG vaccination, sens...

Method used

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  • Serum metabolism marker related to pulmonary tuberculosis and application thereof
  • Serum metabolism marker related to pulmonary tuberculosis and application thereof
  • Serum metabolism marker related to pulmonary tuberculosis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Screening of serum metabolic markers:

[0057] 1. Take 15 healthy people without tuberculosis (Mtb) infection (Un), 16 latent tuberculosis infections (LT), and 17 patients who were newly diagnosed with pulmonary tuberculosis and did not receive treatment (T0). Store at 80°C. After the samples are collected, they are processed uniformly according to the following methods.

[0058] 2. Serum sample processing:

[0059] Take 100 μL of serum sample and put it in an EP tube, add 400 μL of methanol solution, vortex and shake, stand in an ice bath for 5 min, centrifuge at 15,000 rpm and 4 °C for 10 min, take a certain amount of supernatant and add mass spectrometry grade water to dilute to methanol content of 60v / v%, and placed in a centrifuge tube with a 0.22μm filter at 15000g, 4°C

[0060] After centrifugation for 10 min, the filtrate was collected and injected into LC-MS for analysis.

[0061] 3. Liquid chromatography conditions:

[0062] Column temperature: 40°C, flow...

Embodiment 2

[0081] According to the 7 serum metabolic markers in Table 2, the following methods for judging the patient to be tested as active pulmonary tuberculosis were established:

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Abstract

The invention discloses a serum metabolism marker related to pulmonary tuberculosis and application of the serum metabolism marker related to the pulmonary tuberculosis, and the serum metabolism marker related to the pulmonary tuberculosis comprises at least one of 3-oxopalmitic acid, akebia stem saponin, thiolithocholic acid, 2-decyl furan, (decahydro-4, 8, 8-trimethyl-1, 4-methylene azulene-9-subunit) methanol, D-(+)-camphor and 2-methylamino adenosine, and the serum metabolism marker related to the pulmonary tuberculosis comprises at least one of 3-oxopalmitic acid, akebia stem saponin, thiolithocholic acid, 2-decyl furan, (decahydro-4, 8, 8-trimethyl-1, 4-methylene azulene-9-subunit) methanol, D-(+)-camphor and 2-methylamino adenosine. According to the application, the serum metabolism marker is used for preparing a detection reagent or applied to detection related to pulmonary tuberculosis. The serum metabolism marker is suitable for detection related to pulmonary tuberculosis, the accuracy of the detection result is high, and the detection efficiency can be improved.

Description

technical field [0001] The invention relates to a serum metabolic marker related to pulmonary tuberculosis and an application thereof, belonging to the technical field of biological detection. Background technique [0002] Tuberculosis (TB) is a widespread and in many cases fatal chronic infectious disease caused by Mycobacterium tuberculosis (Mtb) infection. most. A quarter of the global population is infected with Mycobacterium tuberculosis but has no clinical manifestations of TB, and 5-15% of them may develop active pulmonary TB. [0003] Early diagnosis of tuberculosis and timely anti-tuberculosis treatment are of great significance to effectively control the progression of tuberculosis and the spread of Mycobacterium tuberculosis. There are currently limited methods for diagnosing TB infection, mainly including sputum smear, sputum bacterial liquid culture, Mycobacterium tuberculosis skin test (TST), radioactive X-rays, serological antibody antigen immunoassays, and ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/72
CPCG01N30/02G01N30/34G01N30/72
Inventor 巫株华魏文静陈珣珣赵雨川廖庆华张晨晨陈亮郭卉欣徐镠粤王嘉雯梁安棋梁鸿迪
Owner 广东省结核病控制中心
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