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Application of urine biomarker detection reagent in preparation of new coronal pneumonia diagnostic kit

A technology of biomarkers and detection reagents, which is applied in the field of in vitro diagnostic reagents, can solve the problems of high infection risk, irregular sampling, and physical discomfort of sampling personnel, so as to reduce the risk of infection, reduce testing costs, and reduce urine indicators Effect

Pending Publication Date: 2022-06-28
重庆市公共卫生医疗救治中心
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  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the current nucleic acid detection of new coronary pneumonia virus is prone to false negatives and false positives due to the irregular sampling. At the same time, the risk of infection of the samplers is high, and the implementation of invasive operations on the subjects can easily lead to physical discomfort.

Method used

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  • Application of urine biomarker detection reagent in preparation of new coronal pneumonia diagnostic kit

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Embodiment 1

[0027] Embodiment 1 The device of the present invention for diagnosing new coronary pneumonia

[0028] 1) Detection device; the detection device includes a high performance liquid chromatography-time-of-flight mass spectrometer; the detection device has built-in reagents for detecting the following biomarkers in urine: oxoglutaric acid, indoxyl and 2-phenylacetamide ;

[0029] 2) Analysis device;

[0030] The analysis device has a built-in data input port for receiving the detection result of the aforementioned detection device, that is, the abundance value of the biomarker;

[0031] The analysis device has a built-in random forest algorithm, which can distinguish between new coronary pneumonia and non-new coronary pneumonia based on the detection results of oxoglutaric acid, indoxyl and 2-phenylacetamide in the urine of known new coronary pneumonia and non-new coronary pneumonia groups. A binary classification model of pneumonia;

[0032] The analysis device can also subst...

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Abstract

The invention relates to the field of in-vitro diagnostic reagents, in particular to application of a reagent for detecting biomarkers in urine to preparation of a diagnostic kit for new coronal pneumonia. According to the invention, urine is used as a biological sample, so that the collection of a detection sample is greatly facilitated, and the infection risk of new coronavirus is reduced; the collection of the urine is non-invasive, so that the compliance of a person to be detected is also greatly improved; due to the fact that the detected urine indexes are few, the detection cost is remarkably reduced, the detection time is shortened, medical institutions can carry out the method more conveniently, meanwhile, the detected urine indexes are few, and the detection accuracy is equivalent to that of a traditional method. Therefore, the urgent requirement of clinical non-innovative coronary pneumonia examination can be well met.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of a urine biomarker detection reagent in the preparation of a diagnostic kit for novel coronavirus pneumonia. Background technique [0002] New coronavirus pneumonia (Corona Virus Disease 2019, COVID-19), referred to as "new coronary pneumonia", named "2019 coronavirus disease" by the World Health Organization, new coronavirus pneumonia is mainly manifested by fever, dry cough, fatigue, etc., a small number of patients Accompanied by upper respiratory and gastrointestinal symptoms such as nasal congestion, runny nose, and diarrhea. Severe cases mostly develop dyspnea after 1 week, and severe cases rapidly progress to acute respiratory distress syndrome, septic shock, metabolic acidosis that is difficult to correct, coagulation dysfunction, and multiple organ failure. It is worth noting that severe and critically ill patients may have moderate to low fever or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 陈耀凯景一娴王静郑鹏武靖
Owner 重庆市公共卫生医疗救治中心
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