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Application of corn ZmBES1/BZR1-3 gene in increasing yield of plant seeds

A technology for plant seeds and corn, applied in the field of genetic engineering, to achieve broad application prospects and increase the effect of grain width

Active Publication Date: 2022-06-28
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Relatively little research has been done on genes other than photosynthesis

Method used

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  • Application of corn ZmBES1/BZR1-3 gene in increasing yield of plant seeds
  • Application of corn ZmBES1/BZR1-3 gene in increasing yield of plant seeds
  • Application of corn ZmBES1/BZR1-3 gene in increasing yield of plant seeds

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Experimental program
Comparison scheme
Effect test

Embodiment

[0024] 1. Gene cloning and vector construction

[0025] The leaves of five-leaf stage corn B73 seedlings were taken, quick-frozen and ground in liquid nitrogen, and total RNA was extracted using the total RNA extraction kit Trizol (TaKaRa) according to its instructions. with PrimeScript TM RT regeant Kit (Takara), using total RNA as a template to synthesize cDNA. Primer 5.0 was used to design cloning primers, and B73 cDNA was used as the template. The specific PCR primers are shown in Table 1. PrimerStar (Takara Company) high-fidelity enzyme was used for amplification. The PCR amplification reaction system was shown in Table 2. The temperature cycle program was: 94°C, 3min; 98°C, 10s, optimal annealing temperature for 10s; 72°C, 20s; 30 cycles Cycle; 72°C for 5 min; 4°C hold.

[0026] Table 1 ZmBES1 / BZR1-3 cloning primers

[0027]

[0028] Table 2 PCR amplification reaction system

[0029]

[0030] 1.1 Transformation of Arabidopsis vector 35S-ZmBES1 / BZR1-3-eGFP con...

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Abstract

The invention discloses application of a corn ZmBES1 / BZR1-3 gene in increasing the yield of plant seeds, the corn ZmBES1 / BZR1-3 gene is cloned by using corn seedling leaves, the function of the gene is studied from phenotypic characteristics of transgenic arabidopsis thaliana and rice, and a new choice is provided for high-yield breeding of rice, improvement of economic benefits of rice and the like.

Description

technical field [0001] The invention relates to the application of maize ZmBES1 / BZR1-3 gene in increasing plant seed yield, and belongs to the field of genetic engineering. Background technique [0002] Grain production has always been an important content of the people's attention, and increasing grain production is of great significance to my country's economic development and food security. Yield is a complex quantitative trait, which is affected by many factors such as plant type, grain per ear, and growth period. Rice is the main food crop. The formation of rice yield per plant is mainly determined by the number of effective panicles, the number of grains per panicle, and the thousand-grain weight. The thousand-grain weight is the most heritable trait among the yield components, which is determined by grain shape and filling degree. , and the grain shape is determined by grain length, grain width and grain thickness. The existing technologies are mainly the applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/46A01H6/20
CPCC12N15/8261C07K14/415Y02A40/146
Inventor 于好强冯文奇刘媛付凤玲杨青青李晚忱
Owner SICHUAN AGRI UNIV
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