Preparation method of composite immune cell with high killing capability on tumor cells

Abstract

A preparation method of composite immune cells with high killing ability to tumor cells is characterized by comprising the following steps: step 1, collecting peripheral blood, separating out mononuclear cells, culturing the separated mononuclear cells, and reserving suspension cells and adherent cells; step 2, culturing mature DC cells through the adherent cells; step 3, inoculating by using a specific immune cell basic culture solution through the suspension cells to culture a specific immune cell culture system; and step 4, adding the DC cells obtained in the step 2 into the specific immune cell culture system for co-culture.

Description

technical field [0001] The invention relates to obtaining immune cells for adoptive immunotherapy, in particular to a method for preparing composite immune cells with high killing ability to tumor cells. Background technique [0002] Due to the high damage and high mortality of tumors, the research and application of anti-tumor treatment methods have always been the focus of attention. Tumor immunotherapy is the fourth largest clinical therapy for tumor treatment, in which immune cells The therapy has been widely used in China. At present, the most widely carried out in China is the adoptive reinfusion therapy of immune cells. [0003] DC cells: also known as dendritic cells (DC), are the most powerful professional antigen presenting cells (APCs) in the body, which can efficiently absorb, process and present antigens. Immature DCs have strong migration ability, and mature DCs can effectively activate naïve T cells and are at the center of initiating, regulating, and maintai...

AI Technical Summary

Problems solved by technology

[0009] 1. In the existing research, most of the single immune cells or killer cells are used for cell therapy. The defect of this therapy is that one type of immune cell cannot be completely effective against the heterogeneous tumor population, because of its role in identifying The tumor target is relatively single, and can only recognize several targets expressing fixed tumor signal patterns for identification, and the effect of killing tumors is poor

[0010] 2. Traditional single immune cells cannot effectively cooperate in the body, promote the activation of the immune system, activate cell receptors, and inhibit cancer cell inhibitory ligands, resulting in a single immune cell entering the body that is easily inhibited by the tumor microenvironment, prone to aging and apoptosis

[0011] 3. Traditional single immune cells lack specificity, have not been trained by antigen peptides carried by antigen-presenting cells, and traditional peptides have not shown effective activation of DC

Lead to low accuracy and specificity in identifying tumors, and poor killing effect;

[0012] 4. Traditional broad-spectrum immune cells have poor infiltration into solid tumors, and can only wander around the periphery of the tumor, but cannot penetrate deep into the tumor;

[0013] 5. The amplification efficiency of existing cell technology is low, and gene-edited and inactivated K562 cancer cells are often used to stimulate proliferation, which is ethically inhumane and has certain safety risks

[0014] Therefore, it is crucial to establish a compound immune cell system that can effectively kill heterogeneous diseased cell populations of tumors. In order to solve the problem of non-accurate specificity of traditional immune cell therapy, single recognition target, easy aging, short retention in the body, infiltration Poor, low amplification, etc., use cancer cell polypeptide fragments to train antigen-presenting cells to activate NK, NKT, γδT, CTL, Inkt, and CD4 regulatory cells to improve tumor recognition specificity. In addition, use such as CD28, Gardiquimod , CD161, poly, etc. greatly increase the content of gamma interferon and tumor necrosis factor required for the activation of immune cells to kill tumors, and through the verification of the ratio of different immune cells, the immune cells can cooperate with each other to achieve the combination of resisting tumor tolerance Compare

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