Cell transport preserving fluid and application thereof

Abstract

The invention discloses a cell transport preserving fluid and application thereof. The cell transport preserving fluid is prepared from the following components in mass concentration: 0.01 mg / ml to 2.5 mg / ml of glucose, 0.05 mg / ml to 10 mg / ml of sodium chloride, 0.05 mg / ml to 3 mg / ml of potassium chloride, 0.05 mg / ml to 1 mg / ml of calcium chloride, 0.05 mg / ml to 20 mg / ml of sodium lactate, 0.05 mg / ml to 2.5 mg / ml of amifostine, 0.05 mg / ml to 3.0 mg / ml of dexrazolone and the balance of water. The cell transport preserving fluid provided by the invention can maintain the activity of various cells for about 7 days, and has a certain advantage in the aspect of maintenance time compared with other preserving fluids; the cell transportation preserving fluid is liquid used in the transportation process of human cells, the components are simple and easy, all components can be normally input into a human body, no human-derived or animal-derived components exist, and the cell transportation preserving fluid plays a great role in development of cells for clinical treatment towards the drug development direction.

Description

technical field [0001] The invention relates to the fields of life science and medical technology, in particular to a cell transport preservation solution and its application. Background technique [0002] The use of cells in clinical treatment involves multiple processes such as cell collection, culture expansion, cell diversification, and cell transplantation. These processes generally include laboratories and hospitals in the place of implementation, so they will involve the transportation and use of cells between different places. [0003] At present, there are already some patents on cell transportation and preservation solutions, such as CN108770836A and CN109090104A, which are used for the transportation and preservation of various cells. They are basically cell activity preservation solutions with complete chemical components, containing pH buffers and various osmotic pressure stabilizers. And a variety of oxygen free radical scavengers, adding the energy substrates...

AI Technical Summary

Problems solved by technology

[0003] At present, there are already some patents on cell transportation and preservation solutions, such as CN108770836A and CN109090104A, which are used for the transportation and preservation of various cells, basically all of them are cells with complete chemical components Active preservation solution, containing pH buffer solution, various osmotic pressure stabilizers and various oxygen free radical scavengers, added energy substrates required by cells to maintain metabolism, the composition is relatively complex, and the cell viability can be maintained for 3-5 days

Generally speaking, the ingredients are relatively complex and the cost is relatively high. They have limited role in promoting the simplification of cell clinical treatment. Most of the components in these preservation solutions cannot enter the human body, and they have limited effect on the development of cell clinical treatment in the direction of medicine, especially for pasting. In the future clinical treatment of wall-cultured cells, professionals have to perform enzyme digestion and use centrifuges, ultra-clean benches and other equipment, which greatly hinders the convenient use of cells in clinical treatment like medicines

Images