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Kit for diagnosing malnutrition condition through flora detection of colon cancer tumor patient

A technology for malnourished and tumor patients, applied in microorganism-based methods, microbial assays/tests, resistance to vector-borne diseases, etc., can solve abnormal defecation, blood-colored stools, and colon cancer patients affecting patient prognosis and treatment resistance. Acceptability and other issues to achieve accurate detection results

Pending Publication Date: 2022-06-07
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Colon cancer is a malignant tumor that originates in the epithelium of the colonic mucosa. Patients with colorectal cancer generally have the following manifestations: 1) Abnormal defecation: When abnormal lesions occur in the colon, the patient will experience abnormalities such as diarrhea and constipation. With blood; 2) Abdominal pain: During the course of colon cancer, the tumor will grow rapidly in the intestinal cavity, which will block the intestinal cavity, causing partial intestinal obstruction or complete intestinal obstruction, and metabolites cannot be excreted and accumulate In the intestinal tract, severe abdominal pain occurs; 3) Inexplicable nausea: The intestinal tract is stimulated by damage, causing nausea, abdominal distension, etc.; 4) Malnutrition: The growth of the tumor needs to consume the energy in the patient's body, coupled with the growth of cancer cells Continuous stimulation makes the patient's digestive function and absorption function worse; the body cannot get more nutrients, weight loss, and symptoms such as dizziness and palpitations;
[0003] Malnutrition in patients with colon cancer can seriously affect the prognosis and treatment tolerance of patients, and greatly reduce the quality of life of cancer patients; It is very important; the current methods for diagnosing the nutritional status of colon cancer patients are mainly blood drawing, consultation, and physical examination; how to construct an accurate, fast, and non-invasive diagnostic method for the nutritional status of colon cancer patients is a problem that needs to be solved now

Method used

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  • Kit for diagnosing malnutrition condition through flora detection of colon cancer tumor patient
  • Kit for diagnosing malnutrition condition through flora detection of colon cancer tumor patient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Study on the intestinal flora of colon cancer under different nutritional status;

[0038] Nutritional status assessment methods and standards: nutritional status is assessed through the PG-SGA assessment tool. PG-SGA includes patient self-assessment sections (including body weight, food intake, symptoms, activity and physical function) and medical staff assessment sections (including related to nutrition). related disease status, metabolic status, and physical examination); the results of the qualitative score are as follows; 0-1 is well-nourished (PG-SGA1), 2-3 is suspected malnutrition (PG-SGA2), and 4-8 is moderate Severe malnutrition (PG-SGA3), ≥ 9 is classified as severe malnutrition (PG-SGA4);

[0039] The diagnostic power of classification and prediction of different microbiota species was examined according to the ROC curve, and the results were as follows: figure 1 Shown in A and 1B; the obtained results show that the diagnostic accuracy of Atopobium.Vaginae,...

Embodiment 2

[0044] Use of the kit for the detection of microbiota in patients with colon cancer and diagnosing malnutrition:

[0045] 1. Extract DNA template

[0046] The feces of colon cancer patients were sampled and stored in DNAhelper protective solution, and the ratio of fecal samples to DNAhelper protective solution was 1:2; genomic DNA was extracted.

[0047] 2. PCR amplification reaction

[0048] Use the PCR reaction kit from the detection kit, which contains the following primer pairs:

[0049] 1) Atopobium.Vaginae DNA forward primer:

[0050] 5'-GGTGAAGCAGTGGAAACACT-3'

[0051] Atopobium.Vaginae DNA reverse primer:

[0052] 5'-ATTCGCTTCTGCTCGCGCA-3'

[0053] 2) Selenomonassputigena DNA forward primer:

[0054] 5'-AGAGTTTGATCCTGGCTCAG-3'

[0055] Selemonassputigena DNA Reverse Primer:

[0056] 5'-CTCAATATTCTCAAGCTCGGTT-3'

[0057] 3) Faecalibacterium prausnitzii DNA forward primer:

[0058] 5'-CCCGGCATCGGGTAGAG-3'

[0059] Faecalibacteriumprausnitzii DNA reverse primer...

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Abstract

The invention discloses a kit for detecting and diagnosing malnutrition conditions of colon cancer tumor patients by flora, which comprises DNA (deoxyribonucleic acid) primer sequences of strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains and DNA primer sequences of the strains, the kit further comprises an excrement sample preservation reagent, a PCR reaction reagent, a PCR product purification reagent and a sequencing reaction reagent. The method comprises the following steps: carrying out DNA extraction and PCR amplification on feces of a patient suffering from the colon cancer, then carrying out sequencing by using DNA sequencing primers of Atobium.Vaginae, Selenomonas sputigena and Faecalibacterium prausnitzii to obtain DNA sequence abundance of three strains in a sample, and diagnosing the nutritional status of the patient suffering from the colon cancer according to the correlation between the abundance of the three strains and the nutritional evaluation condition. The kit diagnosis method provided by the invention can noninvasively and accurately diagnose and evaluate the nutrition status of the colon cancer.

Description

technical field [0001] The invention belongs to the technical field of bacterial flora detection in colon cancer patients, in particular to a kit for detecting and diagnosing malnutrition status of colon cancer tumor patients. Background technique [0002] Colon cancer is a malignant tumor originating from the colonic mucosal epithelium. Patients with colorectal cancer generally have the following symptoms: 1) Abnormal defecation: when the colon has abnormal lesions, the patient will experience abnormal conditions such as diarrhea and constipation. 2) Abdominal pain: During the course of colon cancer, the tumor will grow rapidly in the intestinal lumen, which will block the intestinal lumen, causing partial or complete intestinal obstruction, and metabolites cannot be excreted and accumulated. In the intestine, severe abdominal pain occurs; 3) Inexplicable nausea: the intestine is stimulated by damage, nausea, abdominal distention, etc.; 4) Malnutrition: tumor growth needs t...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6869C12N15/11C12R1/01
CPCC12Q1/689C12Q1/6869C12Q2600/112C12Q2600/166C12Q2531/113C12Q2535/122C12Q2545/101Y02A50/30
Inventor 徐超姚庆华
Owner ZHEJIANG CANCER HOSPITAL
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