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Rapid seedling raising method for tissue culture of dionaea muscipula and application

A Venus flytrap, tissue culture technology, applied in application, horticultural methods, botanical equipment and methods, etc., can solve the problems of red leaves, long cultivation period, easy to produce dead seedlings, etc., to increase the proliferation coefficient, cycle The effect of short and shortened culture period

Active Publication Date: 2022-06-03
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the existing tissue culture technology of Venus flytrap still has the situation that the color of the leaves turns red during the cultivation process, the cultivation period is long, and the seedlings are prone to stiff seedlings during the cultivation process.

Method used

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  • Rapid seedling raising method for tissue culture of dionaea muscipula and application

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for quick seedling raising by tissue culture of Venus flytrap, the steps include:

[0024] 1. Explant cleaning: Select healthy Venus flytrap leaves without pests and diseases as explants, use a soft brush dipped in saturated soap solution to repeatedly brush the surface of the leaves 6 times, and wash the surface soap solution with tap water, and then add 2 Drop Tween-80, soak for 30 minutes, and then rinse with tap water for 60 minutes.

[0025] 2. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect them with 75% (v / v) alcohol for 10s, rinse twice with sterile water, and disinfect with 4% hydrogen peroxide solution for 6 minutes. Rinse the bacteria water twice, then adjust the ultrasonic wave to 25kHz in the ultrasonic cleaner, ultrasonically sterilize with 0.4% sanjierzin solution for 3 minutes, rinse with sterile water for 3 times, and then continue to use 0.02% mercury chloride in the ultrasonic cleaner. The soluti...

Embodiment 2

[0032] A method for quick seedling raising by tissue culture of Venus flytrap, the steps include:

[0033] 1. Explant cleaning: Select healthy Venus flytrap leaves without pests and diseases as explants, use a soft brush dipped in saturated soap solution to lightly brush the surface of the leaves for 8 times, and wash the surface soap solution with tap water, and then dropwise add 3 Drop Tween-80, soak for 40 minutes, and then rinse with tap water for 70 minutes.

[0034] 2. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect them with 75% (v / v) alcohol for 15s, rinse with sterile water for 3 times, and disinfect with 4% hydrogen peroxide solution for 7 minutes. Rinse the bacteria water for 3 times, then adjust the ultrasonic wave to 30kHz in the ultrasonic cleaner, ultrasonically sterilize it with 0.4% Neogel for 2 minutes, rinse with sterile water for 4 times, and then continue to use 0.02% mercuric chloride in the ultrasonic cleaner...

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Abstract

The invention relates to the technical field of insectivorous plant culture, in particular to a dionaea muscipula tissue culture seedling raising method and application. Inducing cluster buds; proliferation of the cluster buds; and rooting the cluster buds. According to the method, during propagation culture of the dionaea muscipula, high-temperature dark culture is carried out at the initial stage of culture, so that a large number of cluster buds can be quickly induced from leaves, the temperature is reduced to the proper temperature in the middle stage, the dark culture is continued, the leaves of the cluster buds can be quickly expanded, and the propagation coefficient is increased, and the proper-temperature and light-dark alternate culture is carried out at the later stage, so that cluster seedlings are more robust, and the survival rate of the dionaea muscipula is increased. On one hand, the number of available leaves is increased, and on the other hand, seedlings are robust during rooting.

Description

technical field [0001] The invention relates to the technical field of carnivorous plant culture, in particular to a fast seedling raising method and application for tissue culture of Venus flytrap. Background technique [0002] Dionaea muscipula, also known as the fly trap, the hell of the flies, etc., is a perennial herb of the sundew family Dionaea muscipula and the only species of the genus Dionaea muscipula, native to the northeast of South Carolina, USA North Carolina, southeastern North Carolina, New Jersey and Virginia, etc., mainly grow in semi-savanna swamps, wetlands around, the soil in this environment is mostly peat and silica sand. Venus flytrap is the most interesting carnivorous plant. Its leaves have evolved into clip-like insect traps, which can attract insects by releasing volatile organic compounds. An action potential is generated and the clip is quickly closed. At the same time, the insect swings repeatedly inside the clip, prompting the clip to secret...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008Y02A50/30
Inventor 张媛张颖王德新邓国宾邱旭向建英
Owner SOUTHWEST FORESTRY UNIVERSITY
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