Composition for inhibiting multiple drug resistance of gram-negative bacteria of poultry and application of composition

A technology for Gram-negative bacteria and multi-drug resistance, which is applied in the direction of antibacterial drugs, active ingredients of heterocyclic compounds, pharmaceutical formulations, etc., can solve the problem of not being able to reduce the resistance of multi-drug-resistant bacteria, and achieve significant synergy, good Market prospect, the effect of improving the antibacterial effect

Pending Publication Date: 2022-05-27
青岛嘉智生物技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Routinely used antibacterial drugs, including the existing compound antibacterial drug products approved by the Ministry of Agriculture, cannot reduce the resistance of multidrug-resistant bacteria

Method used

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  • Composition for inhibiting multiple drug resistance of gram-negative bacteria of poultry and application of composition
  • Composition for inhibiting multiple drug resistance of gram-negative bacteria of poultry and application of composition
  • Composition for inhibiting multiple drug resistance of gram-negative bacteria of poultry and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Test of drug resistance of test strains

[0024] The minimum inhibitory concentration (MIC) is the standard for evaluating whether antibiotics are sensitive to bacteria. The inventors used CLSI (VET01-A4, 2013) recommended micro-broth dilution method to determine the MIC of each strain against conventional veterinary antibacterial drugs; 10 strains were selected, and the screened strains were used as test strains for subsequent experiments. The MIC results of conventional antibiotics on the screened Escherichia coli test strains and Escherichia coli standard strains (ATCC-25922) are shown in Table 1. Conventional antibiotics against the screened Riemerella anatipestifer test strains and standard Riemerella anatipestifer Table 2 shows the MIC results of the Bacillus standard strain (ATCC-11845).

[0025] Table 1

[0026]

[0027]

[0028] It can be seen from the experimental results in Table 1 that the MICs of the 10 strains of Escherichia coli screene...

Embodiment 2

[0032] Example 2 Synergy test of conventional antibiotics in combination

[0033] The 10 multi-drug resistant Escherichia coli test strains screened in Example 1 were used as the test objects, and 30 veterinary antibacterial drug compositions were screened. A certain concentration of MIC value is combined in the 96-well reaction plate by column and row respectively (the concentration of the row is generally set to 1, 1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64, 1 / 128 times the MIC, the serial concentration is generally set to 1, 1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64 times the MIC), and the inoculum volume is 1×10 6 The bacterial suspension of CFU / ml was incubated aerobically in a 37°C incubator for 18-24 hours, and the results were observed. Each group of experiments was repeated at least twice. The test results are shown in Table 3.

[0034] table 3

[0035]

[0036]

[0037]

[0038] From the test results in Table 3, it can be seen that the synergistic effect of the 30 two-drug c...

Embodiment 3

[0039] Embodiment 3 Ampicillin and potassium clavulanate synergy test

[0040] Taking the Escherichia coli test strain screened in Example 1 as the test object, the chessboard microdilution method was used to combine the MIC values ​​of the corresponding strains of ampicillin and clavulanate potassium on a 96-well reaction plate in a column and a row respectively. (The row concentration is generally set to 1, 1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64, 1 / 128 times the MIC, and the column concentration is generally set to 1, 1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64 times MIC), the inoculum volume is 1×10 6 The bacterial suspension of CFU / ml was incubated aerobically in a 37°C incubator for 18-24 hours, and the results were observed. Each group of experiments was repeated at least twice. The experimental results are shown in Table 4.

[0041] Determination of results: The partial inhibitory concentration FICI index was used as the basis for the determination of the combined drug susceptibility te...

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Abstract

The invention provides a composition for inhibiting multiple drug resistance of gram-negative bacteria of poultry and application thereof, the composition comprises potassium clavulanate and an antibacterial drug A or pharmaceutically acceptable salt of the antibacterial drug A, and the antibacterial drug A is ampicillin or ceftiofur. The composition has a remarkable synergistic effect on gram-negative drug-resistant bacteria such as escherichia coli and riemerella anatipestifer, the MIC (minimal inhibitory concentration) of an in-vitro single drug can be reduced by 8-64 times, the antibacterial effect can be remarkably improved, and the composition has a relatively good market prospect when being used for inhibiting infection of multidrug-resistant bacteria in poultry breeding.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicine, and particularly relates to a composition for inhibiting multidrug resistance of poultry Gram-negative bacteria and its application. Background technique [0002] In order to strengthen the management of veterinary antibiotics and effectively regulate the use of veterinary drugs in the breeding process, the Ministry of Agriculture organized and issued the "Administrative Measures for the Clinical Application of Veterinary Antimicrobials for Food Animals", which stipulates that the legal varieties of antibiotics that can be used in poultry breeding are approximately There are 45 kinds, and in order to control the residues of veterinary drugs in food animals, there are currently about 20 kinds of antibacterial drugs routinely used in large-scale breeding groups. The widespread and overuse of these drugs in poultry feeding and disease treatment has led to the emergence of multidrug-resis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/43A61K31/546A61K31/424A61P31/04
CPCA61K31/424A61K31/43A61K31/546A61P31/04A61K2300/00Y02A50/30
Inventor 张瑞丽秦立廷吕闯刁硕于明明魏笑笑戴荣莲
Owner 青岛嘉智生物技术有限公司
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