Method for metabonomics sample component detection

Abstract

The invention provides a metabonomics sample component detection method, which comprises: 1, carrying out separation purification on a first to-be-detected sample to obtain a single-component second to-be-detected sample; 2, placing the second to-be-tested sample on a diamond chip, and externally applying a magnetic field to perform polarization treatment so as to obtain a third to-be-tested sample with consistent spinning direction; 3, performing nuclear magnetic resonance scanning on the third to-be-detected sample by taking the NV color center in the diamond chip as a probe so as to obtain structural information of the third to-be-detected sample; and 4, carrying out mass spectrum quantitative analysis on the third to-be-detected sample subjected to nuclear magnetic resonance scanning. Therefore, non-targeted qualitative and quantitative analysis of various low-abundance unknown samples in complex samples can be realized, an instrument system is simple, repeatability and reproducibility are good, and sensitivity is high.

Description

technical field [0001] The present application relates to the field of analysis and detection, in particular, to a method for component detection of metabolomics samples. Background technique [0002] Metabonomics / metabolomics is a research method that imitates the research ideas of genomics and proteomics, quantitatively analyzes all metabolites in the organism, and finds the relative relationship between metabolites and physiological and pathological changes. It is a research method of systems biology. component. Most of its research objects are small molecular substances with a relative molecular mass of less than 1000. Advanced analysis and detection technology combined with computational analysis methods such as pattern recognition and expert systems is the basic method of metabolomics research. The research methods of metabolomics generally have two methods: One method is called metabolite fingerprinting, which uses liquid chromatography-mass spectrometry (LC-MS) met...

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Problems solved by technology

However, Griffin can only detect high-abundance metabolites with NMR, and cannot analyze low-abundance metabolites due to the low sensitivity of NMR detection.

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