Method for detecting content of sialic acid in milk powder or whey powder
A detection method, whey powder technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of sialic acid content in unpublished whey powder and formula milk powder, achieve high accuracy, improve detection efficiency, and operate simple effect
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Embodiment 1
[0053] In this embodiment, a commercially available whey powder rich in casein glycomacropeptide (hereinafter referred to as CGMP), α-lactalbumin powder, and whey powder rich in milk fat globule membrane (hereinafter referred to as MFGM) were selected to conduct saliva Acid content detection. The specific method is as follows:
[0054] (1) Reagents:
[0055] Water: Class I water in accordance with GB / T 6682;
[0056] Sulfuric acid: superior grade;
[0057] Anhydrous sodium acetate: superior grade;
[0058] 50% sodium hydroxide: chromatographically pure.
[0059] (2) Sample pretreatment:
[0060] Accurately weigh 0.1g (accurate to 0.0001g) of whey powder rich in casein glycomacropeptide, dissolve it in water and set the volume in a 10ml volumetric flask, vortex and shake well; take 1mL of the dissolved liquid in a 15mL centrifuge tube In the process, use sulfuric acid solution (0.05mol / L) to fix the volume to 10mL; place the prepared hydrolyzate in a constant temperature ...
Embodiment 2
[0086] In this example, a commercially available brand of infant formula powder 1, infant formula powder 3 and whole milk powder were selected for the detection of sialic acid content. The specific method is as follows:
[0087] (1) Reagents:
[0088] Water: Class I water in accordance with GB / T 6682;
[0089] Sulfuric acid: superior grade;
[0090] Sodium chloride, sodium acetate, 50% sodium hydroxide: all chromatography grade.
[0091] Sample pretreatment:
[0092]Accurately weigh 1g of the milk powder sample (accurate to 0.0001g), dissolve it in water and dilute it in a 10mL colorimetric tube, and vortex to mix. Take 2 mL of the dissolved liquid sample in a 15 mL centrifuge tube, and dilute to 10 mL with sulfuric acid solution (0.05 mol / L). Place the prepared milk powder hydrolyzate in a constant temperature water bath and adjust the temperature of the water bath. During this process, use a mercury thermometer inserted into the hydrolyzate for actual measurement until ...
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