Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bionic drug-loaded nanoparticles for specific targeting pulsed electric field ablation postoperative inflammatory region and preparation method thereof

A pulsed electric field and drug-loaded nanotechnology, applied in the field of biomimetic drug-loaded nanoparticles and preparation, can solve the problems of high cost, complicated preparation process, and expensive activation steps, and achieve low cost, high safety, and medical transformation prospects. The effect of short preparation cycle

Pending Publication Date: 2022-05-13
ZHEJIANG UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, since neutrophil membrane biomimetic nanomaterials are generally used for anti-tumor therapy, the existing preparation methods often require a series of activation processes for neutrophil membranes to make them possess immune properties, and the preparation process is relatively complicated, and The activation step is costly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bionic drug-loaded nanoparticles for specific targeting pulsed electric field ablation postoperative inflammatory region and preparation method thereof
  • Bionic drug-loaded nanoparticles for specific targeting pulsed electric field ablation postoperative inflammatory region and preparation method thereof
  • Bionic drug-loaded nanoparticles for specific targeting pulsed electric field ablation postoperative inflammatory region and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] figure 1 It is a schematic flowchart of a method for preparing biomimetic drug-loaded nanoparticles specifically targeting the inflammatory area after pulsed electric field ablation provided by an embodiment. The preparation method of the biomimetic drug-loaded nanoparticles specifically targeting the inflammatory area after pulsed electric field ablation provided in this example includes the following steps:

[0037]Choose mice or rats. In this example, mice are used. After the mice are anesthetized with 5% chloral hydrate, blood is taken from the heart in an EP tube added with heparin. Take a 50ml centrifuge tube, carefully add mouse peripheral blood neutrophil separation solution according to the kit instructions, then carefully add blood samples, and centrifuge at 500g for 25min. After centrifugation, carefully absorb the neutrophil layer of the lower layer of cells, then add 3 times the volume of red blood cell lysate, mix gently by pipetting, lyse for 10 minutes,...

Embodiment 2

[0041] The difference between this example and Example 1 is that DPPC, CH, and DSPC are completely dissolved in chloroform at a molar ratio of 5:2:1, and ultrasonicated in a water bath at 60°C for a period of time.

Embodiment 3

[0043] The drug-loaded liposomes, neutrophil membranes and neutrophil membrane biomimetic drug-loaded nanoparticles obtained in Example 1 were detected by transmission electron microscopy. The results of transmission electron microscopy are shown in figure 2 .

[0044] The results showed that the electron micrograph of the drug-loaded liposome was figure 2 In a, it can be seen that liposomes are round and uniform in particle size, and the dispersion system is good; the electron microscope image of neutrophil cell membrane is figure 2 In b, the double-layer cell membrane structure can be seen; the electron microscope image of the neutrophil membrane biomimetic drug-loaded nanoparticles is figure 2 In c, it can be seen that the round drug-loaded nanoparticles are wrapped in the double cell membrane.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a bionic drug-loaded nanoparticle for a specific targeted pulsed electric field ablation postoperative inflammatory region and a preparation method. The bionic drug-loaded nanoparticle comprises a drug-loaded liposome and a neutrophile granulocyte membrane coated on the surface of the drug-loaded liposome. The preparation method comprises the following steps: obtaining a neutrophile granulocyte membrane from activated neutrophile granulocytes through an osmotic pressure gradient method, then mixing the neutrophile granulocyte membrane with a drug-loaded liposome, and treating the obtained mixture through a nano extruder to obtain the neutrophile granulocyte membrane drug-loaded nanoparticles. The neutrophile granulocyte membrane targeting drug-loading nanoparticles with targeting ability are prepared by creatively wrapping the neutrophile granulocyte membrane on the surface of the drug-loading liposome by utilizing the characteristics that neutrophile granulocyte has high expelling ability on an inflammation site and can gather on the inflammation site. Based on the principle that neutrophile granulocytes are naturally collected to an inflammatory site after pulsed electric field ablation, a focus in an inflammatory state is targeted, and drugs are released, so that the focus is precisely targeted and treated.

Description

technical field [0001] The application relates to the fields of cell therapy and neutrophil membrane biomimetic nano-medicine, in particular to a biomimetic drug-loaded nano-particle specifically targeting the inflammatory area after pulsed electric field ablation and a preparation method. Background technique [0002] In recent years, with the development of cell electrophysiology, a new type of pulsed electric field ablation technology has emerged. Different from traditional ablation techniques (radiofrequency ablation, microwave ablation, laser ablation, etc.), pulsed electric field ablation acts on the tumor cell membrane through extremely short but strong electrical pulses, forming irreversible "nanoscale" pores on the cell membrane, which leads to the imbalance of cell homeostasis , induce tumor cell apoptosis, and more importantly, the skeleton components that constitute blood vessels, nerves, and pancreaticobiliary ducts can be preserved because they do not contain l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K45/00A61P29/00C12N5/0787
CPCA61K9/5068A61K45/00A61P29/00C12N5/0642A61K9/127C12N2509/00
Inventor 蒋天安谢丽婷许敏许丹霞赵齐羽
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com