Biological organic selenium earthworm selenium and preparation method thereof
A technology of organic selenium and earthworms, which is applied in the field of livestock and poultry breeding, can solve the problems that sodium selenite is easy to remain and pollute the environment, the addition of low concentration cannot be achieved, and the industrialization of earthworm selenium enrichment is difficult, etc., so as to achieve short production cycle and safe High performance and high conversion rate
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Embodiment 1
[0032] Step 1: Use cow dung, bean dregs, and beer's grains to prepare earthworm feed and cultivate earthworms. The mass fraction of cow dung, bean dregs, and beer's grains is 35:28:37. Ferment for 1.5 days, breed for 15 to 20 days, and collect earthworms by phototactic method.
[0033] Step 2: Add 1800 mg / kg sodium selenite aqueous solution of earthworm body weight to the earthworms, the mass fraction ratio of earthworms and sodium selenite aqueous solution is 90:10, wherein the analytical purity of sodium selenite is more than 98%, and the reaction time is 0.75h at 31°C .
[0034] Step 3: Earthworm enzymatic hydrolysis was started with earthworm enzyme at 57.8°C for 0.7h; enzymatic hydrolysis at 48.5°C was liquefied for 3.8 h, and enzymatic hydrolysis was continued at 36.5°C for 22h.
[0035] Step 4: purify earthworm selenium, cool earthworm selenium to room temperature 26°C, filter three times with double-layer gauze cloth, take the supernatant, and discard the residue in th...
Embodiment 2
[0039] The difference between this example and Example 1 is that: Step 2: after adding the sodium selenite aqueous solution to the earthworms, act at 37° C. for 0.20 h.
Embodiment 3
[0041] The difference between this embodiment and embodiment 1 is: after the sodium selenite aqueous solution is added to the earthworms in the second step, the pre-action is not performed, and the third step is directly entered.
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