Application of piperine in preparation of bacteriostatic agent for preventing and treating tomato pathogenic bacteria

A technology of tomato botrytis cinerea and piperine, which is applied in the field of agricultural biology and achieves the effects of wide acting sites, less resistance and low residue.

Pending Publication Date: 2022-05-13
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the effectiveness of piperine on plant pathogens

Method used

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  • Application of piperine in preparation of bacteriostatic agent for preventing and treating tomato pathogenic bacteria
  • Application of piperine in preparation of bacteriostatic agent for preventing and treating tomato pathogenic bacteria
  • Application of piperine in preparation of bacteriostatic agent for preventing and treating tomato pathogenic bacteria

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Experimental program
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Embodiment 1

[0028] 1. Botrytis cinerea, provided by Green Pesticide Research Institute of Qingdao Agricultural University, preserved at 4°C. Transfer the preserved strains to a culture plate for activation, put them in a constant temperature incubator and cultivate them at 25°C until the mycelia cover the entire plate, and use a puncher to punch a 5mm-diameter bacteria cake along the outer edge of the colony. spare.

[0029] 2. Accurately weigh 20 mg of piperine, dissolve it in DMSO, and prepare a 20,000 mg / L piperine stock solution.

[0030] 3. Determination of antibacterial activity by growth rate method.

[0031] Add the prepared piperine stock solution to the PDA medium (about 50°C) to form a drug-containing medium with a concentration of 200, 150, 100, 50, 25, and 12.5 mg / L, shake well and pour the plate . Take the culture medium with the same dose of DMSO as a control, inoculate d=5mm purely cultured hyphae discs of cinerea cinerea in the center of the plate, culture at a constan...

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PUM

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Abstract

The invention discloses application of piperine in preparation of a bacteriostatic agent for preventing and treating tomato pathogenic bacteria. According to the application disclosed by the invention, the specific action part of the piperine in the process of inhibiting the botrytis cinerea is explored, and the piperine is verified to be capable of obviously inhibiting the spore growth and germination of the botrytis cinerea, changing the ultrastructure of thalli, inhibiting the enzyme activity of SOD, POD, CAT, SDH and NAD-MDH related to the thalli and obviously inhibiting the morbidity of tomato infection with the botrytis cinerea. Therefore, the piperine can effectively inhibit the growth of botrytis cinerea and is used for preparing a natural source bactericide.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to the application of piperine in the preparation of antibacterial agents for preventing and treating tomato pathogenic bacteria. Background technique [0002] Tomato is the vegetable with the second largest planting area in the world, and it is also an important economic crop in my country. Botrytis cinerea is a worldwide tomato disease. It is characterized by rapid transmission and serious damage. It is the most serious and frequent disease on tomatoes. The disease mainly damages fruits and causes fruit rot, and can also damage leaves and stems. Hehua has greatly affected the tomato production, and the production reduction can reach 20%-30% in severe cases, which brings huge economic losses to farmers. [0003] At present, there are many studies on the isolation and antibacterial effect of alkaloid compounds in plants at home and abroad, and reports have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/40A01P3/00
CPCA01N43/40
Inventor 包华谢睿睿李康郝智慧
Owner QINGDAO AGRI UNIV
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